CAS OpenIR  > 生化工程国家重点实验室
Determination of the nucleosides and nucleobases in Tuber samples by dispersive solid-phase extraction combined with liquid chromatography-mass spectrometry
Liu, Ping1; Li, Yuan-Yuan1,4; Li, Hong-Mei1; Wan, Duan-Ji1; Tang, Ya-Jie1,2,3; Tang, YJ
2011-02-21
Source PublicationANALYTICA CHIMICA ACTA
ISSN0003-2670
Volume687Issue:2Pages:159-167
AbstractA simple, fast and inexpensive method based on dispersive solid phase extraction (DSPE) combined with LC-MS was developed for simultaneous determination of 7 nucleosides and nucleobases (i.e., adenine, hypoxanthine, uridine, adenosine, guanine, guanosine, and inosine) in Tuber fruiting-bodies and fermentation mycelia. The DSPE procedure was firstly introduced to remove the protein interference from sample solutions, and D3520 macroporous resin was chosen as the DSPE sorbent because of its high removal capability on protein interferences, but low adsorption rate on analytes. Besides, key parameters on DSPE procedure (i.e., macroporous resin type, macroporous resin amount, methanol concentration, and vortex time) were optimized, and the protein removal efficacy could achieve about 95% after the process optimization. Though the method validation test, the DSPE-LC-MS method was confirmed to be precise, accurate and sensitive, and the column blinding problem was solved successfully. By using this established method, the total amount of nucleosides and nucleobases in the fermentation mycelia was determined to range from 4881.5 to 12,592.9 mu g g(-1), which was about 2-25 times higher than the fruiting-bodies (from 498.1 to 2274.1 mu g g(-1)). The formulation of nucleosides and nucleobases in the fermentation mycelia maintained relatively constant, while the formulation in Tuber fruiting-bodies varied significantly with their species. Hierarchical cluster analysis (HCA) showed the formulation similarity of nucleosides and nucleobases between Tuber fermentation mycelia and the fruiting-bodies of Tuber indicum and Tuber himalayense. From the viewpoint of nucleosides and nucleobases, this work confirms the potentiality of Tuber fermentation mycelia as the alternative resource for its fruiting-bodies. (C) 2010 Elsevier B.V. All rights reserved.
KeywordTruffle Nucleosides Nucleobases Protein Removal Dispersive Solid-phase Extraction Liquid Chromatography-mass Spectrometry (Lc-ms)
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1016/j.aca.2010.12.025
Indexed BySCI
Language英语
WOS KeywordBETA-LACTAM ANTIBIOTICS ; MACROPOROUS RESINS ; POLYSACCHARIDES PRODUCTION ; CAPILLARY-ELECTROPHORESIS ; PESTICIDE-RESIDUES ; CULTURED CORDYCEPS ; CELL-GROWTH ; BOVINE-MILK ; FERMENTATION ; SINENSE
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000287564700008
Citation statistics
Cited Times:41[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5087
Collection生化工程国家重点实验室
Corresponding AuthorTang, YJ
Affiliation1.Hubei Univ Technol, Coll Bioengn, Hubei Prov Key Lab Ind Microbiol, Minist Educ,Key Lab Fermentat Engn, Wuhan 430068, Peoples R China
2.Chinese Acad Sci, Shanghai Adv Res Inst, Lab Biorefinery Engn, Shanghai 201203, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
4.City Univ Hong Kong, Dept Biol & Chem, Res Grp Bioact Prod, Kowloon, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Liu, Ping,Li, Yuan-Yuan,Li, Hong-Mei,et al. Determination of the nucleosides and nucleobases in Tuber samples by dispersive solid-phase extraction combined with liquid chromatography-mass spectrometry[J]. ANALYTICA CHIMICA ACTA,2011,687(2):159-167.
APA Liu, Ping,Li, Yuan-Yuan,Li, Hong-Mei,Wan, Duan-Ji,Tang, Ya-Jie,&Tang, YJ.(2011).Determination of the nucleosides and nucleobases in Tuber samples by dispersive solid-phase extraction combined with liquid chromatography-mass spectrometry.ANALYTICA CHIMICA ACTA,687(2),159-167.
MLA Liu, Ping,et al."Determination of the nucleosides and nucleobases in Tuber samples by dispersive solid-phase extraction combined with liquid chromatography-mass spectrometry".ANALYTICA CHIMICA ACTA 687.2(2011):159-167.
Files in This Item:
File Name/Size DocType Version Access License
20131107-1001-复件 1-s(742KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, Ping]'s Articles
[Li, Yuan-Yuan]'s Articles
[Li, Hong-Mei]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, Ping]'s Articles
[Li, Yuan-Yuan]'s Articles
[Li, Hong-Mei]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, Ping]'s Articles
[Li, Yuan-Yuan]'s Articles
[Li, Hong-Mei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.