CAS OpenIR
Preventive effect of swim bladder hydrolysates on cyclophosphamide-induced ovarian injury in mice
Ge, Enhui1; Zha, Rui1,2; Gao, Qing1,2; Guo, Lirong1; Wang, Binglong1; Lin, Qiqi1; Zhang, Guifeng3; Xie, Weiquan4,5; Yin, Hui1; Liu, Tao1
2021-12-01
Source PublicationJOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
ISSN1341-8076
Pages11
AbstractAims This study aimed to prepare swim bladder hydrolysate (SBH) with M-n < 4000 Da, and investigate its effects on cyclophosphamide (CTX)-mediated ovarian injury in mice. Methods Hydrolysates were prepared by heating extraction, enzymatic hydrolysis and ultrafiltration. M-n and distribution of SBH were analyzed via gel filtration chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Changes in the mouse oestrus cycle were determined by cytological examination. The number of follicles was examined using histopathology. Enzyme-linked immunosorbent assays (ELISAs) were used to determine the serum sex hormone levels. Results The M-n of SBH, prepared by heating extraction, enzymatic hydrolysis, ultrafiltration, and from different batches, was below 4000 Da, and the preparation process was stable. Compared with the control group, the low-, middle-, and high-dose SBH treatment groups showed different trends in oestrus duration, serum sex hormone levels, and the number of primordial and secondary follicles. The oestrus cycle duration of the high-dose SBH group was longer than that of the model group. The serum luteinizing hormone, follicle-stimulating hormone, and anti-Mullerian hormone levels in the middle-dose group were the closest to those of control group. The number of primordial and secondary follicles in the medium-dose group was significantly higher than that in the model group and closest to those of control group. Conclusion After heating extraction, trypsin/Flavourzyme hydrolysis and ultrafiltration, a hydrolysate with M-n below 4000 Da could be prepared. We found that a moderate (400 mg/kg) SBH dose resulted in the greatest effect on ovarian injury remission in mice.
Keywordfemale infertility nutrition ovarian function pharmacology postpartum care
DOI10.1111/jog.15122
Language英语
WOS KeywordANTI-MULLERIAN HORMONE ; GRANULOSA-CELLS ; CHEMOTHERAPY ; FAILURE ; WOMEN ; ANTIOXIDANT ; PREMATURE ; TOXICITY ; RESERVE ; PROTEIN
Funding ProjectNational Natural Science Foundation of China[81770527] ; National Natural Science Foundation of China[82171700] ; Natural Science Foundation of Guangdong[2017A020214020] ; Middle-aged and Young Teachers' Basic Ability Promotion Project of Guangxi[2020KY12033]
WOS Research AreaObstetrics & Gynecology
WOS SubjectObstetrics & Gynecology
Funding OrganizationNational Natural Science Foundation of China ; Natural Science Foundation of Guangdong ; Middle-aged and Young Teachers' Basic Ability Promotion Project of Guangxi
WOS IDWOS:000724446300001
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/51279
Collection中国科学院过程工程研究所
Corresponding AuthorLiu, Tao
Affiliation1.Guangdong Pharmaceut Univ, Sch Biosci & Biopharmaceut, Guangdong Prov Key Lab Pharmaceut Bioact Subst, Guangzhou 510006, Peoples R China
2.Guangdong Pharmaceut Univ, Sch Pharm, Guangzhou, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
4.Univ South China, Inst Pharm & Pharmacol, Hengyang, Peoples R China
5.Guilin Med Univ, Sch Pharm, Guilin, Peoples R China
Recommended Citation
GB/T 7714
Ge, Enhui,Zha, Rui,Gao, Qing,et al. Preventive effect of swim bladder hydrolysates on cyclophosphamide-induced ovarian injury in mice[J]. JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH,2021:11.
APA Ge, Enhui.,Zha, Rui.,Gao, Qing.,Guo, Lirong.,Wang, Binglong.,...&Liu, Tao.(2021).Preventive effect of swim bladder hydrolysates on cyclophosphamide-induced ovarian injury in mice.JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH,11.
MLA Ge, Enhui,et al."Preventive effect of swim bladder hydrolysates on cyclophosphamide-induced ovarian injury in mice".JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH (2021):11.
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