CAS OpenIR
Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes
Chu, Hongwei1; Zhao, Qun1; Liu, Ju2; Yang, Kaiguang1; Wang, Yanlei2; Liu, Jianhui1; Zhang, Kun1; Zhao, Baofeng1; He, Hongyan2; Zheng, Yong3; Zhong, Shijun4; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
2021-12-21
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Pages10
AbstractLimited by the rare efficient extraction system in extracting hydrophobic membrane protein complexes (MPCs) without compromising the stability of protein-protein interactions (PPIs), the in-depth functional study of MPCs has lagged far behind. In this study, the first systematic screening of ionic liquids (ILs) was performed and showed that triethylammonium acetate (TEAA) IL exhibited excellent performance in stabilizing PPIs, which was further confirmed by molecular docking simulations. By combining TEAA with the conventional detergent Nonidet P-40 (NP-40), a novel IL-based extraction system, i-TAN (TEAA IL with 1% NP-40), was proposed, which demonstrated superior performance in extracting and stabilizing MPCs, attributed to its larger size, more uniform distribution, and closer-to-neutral microenvironment of micelles. Extraction of MPCs with i-TAN allowed the confident identification of more hydrophobic EGFR-interacting proteins that are easily dissociated during the extraction process. Quantitative analysis of the difference in EGFR complexes between trastuzumab-sensitive and trastuzumab-resistant breast cancer cells provided comprehensive insights to understand the drug resistance mechanism, suggesting that i-TAN has great potential in interactomics and functional analysis of MPCs. This study provides a novel strategy for MPC extraction and downstream processing.
DOI10.1021/acs.analchem.1c03195
Language英语
WOS KeywordEGFR ; STABILIZATION ; TRASTUZUMAB ; LANDSCAPE ; STABILITY ; NETWORKS ; COVERAGE ; SINGLE
Funding ProjectNational Key Research and Development Program of China[2016YFA0501401] ; National Key Research and Development Program of China[2018YFA0507703] ; National Natural Science Foundation[21834006] ; National Natural Science Foundation[21725506] ; National Natural Science Foundation[31770893] ; National Natural Science Foundation[21775150] ; National Natural Science Foundation[21922813] ; CAS Key Project in Frontier Science[QYZDY-SSW-SLH017] ; Youth Innovation Promotion Association, CAS[2017066] ; Youth Innovation Promotion Association, CAS[2020184] ; Cooperation agreement of Innovation Academy for Precision Measurement Science and Technology, CAS
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
Funding OrganizationNational Key Research and Development Program of China ; National Natural Science Foundation ; CAS Key Project in Frontier Science ; Youth Innovation Promotion Association, CAS ; Cooperation agreement of Innovation Academy for Precision Measurement Science and Technology, CAS
WOS IDWOS:000735943400001
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/51529
Collection中国科学院过程工程研究所
Corresponding AuthorZhao, Baofeng; He, Hongyan; Zhang, Lihua
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Multiphase Complex Syst, CAS Key Lab Green Proc & Engn,Beijing Key Lab Ion, Beijing 100190, Peoples R China
3.Beijing Inst Life, Natl Ctr Prot Sci Beijing, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China
4.Dalian Univ Technol, Dalian 116024, Peoples R China
Recommended Citation
GB/T 7714
Chu, Hongwei,Zhao, Qun,Liu, Ju,et al. Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes[J]. ANALYTICAL CHEMISTRY,2021:10.
APA Chu, Hongwei.,Zhao, Qun.,Liu, Ju.,Yang, Kaiguang.,Wang, Yanlei.,...&Zhang, Yukui.(2021).Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.ANALYTICAL CHEMISTRY,10.
MLA Chu, Hongwei,et al."Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes".ANALYTICAL CHEMISTRY (2021):10.
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