CAS OpenIR
Apoferritin nanoparticle based dual-antigen influenza conjugate vaccine with potential cross-protective efficacy against heterosubtypic influenza virus
Sheng, Yanan1,2; Wei, Jiangxue1,2; Li, Zhengjun1; Su, Zhiguo1; Ma, Guanghui1; Zhang, Songping1
2022-05-01
Source PublicationPARTICUOLOGY
ISSN1674-2001
Volume64Pages:56-64
AbstractFerritin nanoparticles with self-assembling properties have been widely explored as vaccine carrier by displaying foreign antigens through genetic fusion strategy. In the present work, an apoferritin (AFt) nanoparticle was tested as influenza vaccine carrier by chemically conjugating a matrix protein 2 ectodomain (M2e) antigen peptide or/and the full-length hemagglutinin (HA) antigen on the outer surface of the AFt, with heterobifunctional sSMCC or SM(PEG)24 containing PEG chain as linkers. To each AFt nanoparticle, about 30-32 M2e or 1.8 HA antigen could be coupled. The AFt-(PEG)(24)-M2e, in which the M2e was coupled through SM(PEG)24 containing PEG chain, conferred higher protective efficacy in immunized mice than AFt-M2e did, but was less effective than AFt-(PEG)(24)-HA. When both M2e and HA were coupled, the synthesized dual-antigen vaccine candidate AFt-(PEG)(24)-M2e/HA elicited high level of M2e and HA antigen-specific antibodies and conferred 100% protection against lethal infection of homologous PR8 H1N1 virus strain and 70% protection against a heterologous A/FM/1/47 (FM1, H1N1) strain, which was more effective than the M2e or HA single antigen vaccine candidates. The potential cross-protective effect of the dual-antigen vaccine was further demonstrated by significant specific hemagglutination inhibition (HAI) titers in serum of the immunized mice against three other heterologous viral strains including A/Singapore/GP1908/2015 (IVR-180) H1N1, A/Anhui/1/2005 H5N1, and A/Hong Kong H3N2. (C) 2021 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V.
KeywordInfluenza vaccine Apoferritin nanoparticle Hemagglutinin M2e Conjugate vaccine
DOI10.1016/j.partic.2021.04.001
Language英语
Funding ProjectNational Natural Science Foundation of China[21821005] ; National Natural Science Foundation of China[31970872]
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Chemical ; Materials Science, Multidisciplinary
Funding OrganizationNational Natural Science Foundation of China
WOS IDWOS:000743069300006
PublisherELSEVIER SCIENCE INC
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/51700
Collection中国科学院过程工程研究所
Corresponding AuthorZhang, Songping
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Sheng, Yanan,Wei, Jiangxue,Li, Zhengjun,et al. Apoferritin nanoparticle based dual-antigen influenza conjugate vaccine with potential cross-protective efficacy against heterosubtypic influenza virus[J]. PARTICUOLOGY,2022,64:56-64.
APA Sheng, Yanan,Wei, Jiangxue,Li, Zhengjun,Su, Zhiguo,Ma, Guanghui,&Zhang, Songping.(2022).Apoferritin nanoparticle based dual-antigen influenza conjugate vaccine with potential cross-protective efficacy against heterosubtypic influenza virus.PARTICUOLOGY,64,56-64.
MLA Sheng, Yanan,et al."Apoferritin nanoparticle based dual-antigen influenza conjugate vaccine with potential cross-protective efficacy against heterosubtypic influenza virus".PARTICUOLOGY 64(2022):56-64.
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