CAS OpenIR
O-GlcNAcylation Quantification Based on Electron-Transfer/Higher-Energy Collisional Dissociation and Its Application in O-GlcNAcylation Site Mapping of Liver Proteomics in High Fat Diet-fed Mice
Shao Wen-Ya1,2; Liang Yu2; Liu Jian-Xi4; Liu Hong-Tao3; Wang Zhao-Wei5; Sui Zhi-Gang2; Zhao Bao-Feng2; Zhang Xiao-Dan2; Liang Zhen2; Zhang Li-Hua2; Zhang Yu-Kui2
2021-12-01
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume49Issue:12Pages:11
AbstractO-linked beta-N-acetylglucosaminylation (O-GlcNAcylation ) is a single carbohydrate moiety post-translational modification that occurs on serine and threonine side chains of intracellular protein and plays an important role in nutrient metabolism. However, the great research challenges are the inherently low stoichiometry, , poor ionization efficiency of O-GlcNAc peptides and no specific animo acid sequence. In this study, a strategy for quantitative detection of O-GlcNAc sites was developed by combining pseudo-isobaric dimethyl strategy, which was applied for the O-GlcNAcylation sites profiling towards the mice liver. In total, 783 O-GlcNAc sites were unambiguously quantified from high-fat fed mice liver. Among which , 122 O-GlcNAc sites were differentially expressed, corresponding to 85 O-GlcNAc proteins. Finally, the biological function of the differentially expressed O-GlcNAc proteins was analyzed.
KeywordO-linked beta-N-acetylglucosaminylation Quantitative proteomics Electron transfer/higher energy collision dissociation
DOI10.19756/j.issn.0253-3820.210623
Language英语
WOS KeywordOGT ; GLYCOPROTEINS ; ACTIVATION
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000753629000001
PublisherSCIENCE PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/51977
Collection中国科学院过程工程研究所
Corresponding AuthorZhang Li-Hua
Affiliation1.Fujian Med Univ, Sch Publ Hlth, Dept Prevent Med, Fuzhou 350122, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
4.Fujian Normal Univ, Coll Environm Sci & Engn, Fuzhou 350007, Peoples R China
5.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Shao Wen-Ya,Liang Yu,Liu Jian-Xi,et al. O-GlcNAcylation Quantification Based on Electron-Transfer/Higher-Energy Collisional Dissociation and Its Application in O-GlcNAcylation Site Mapping of Liver Proteomics in High Fat Diet-fed Mice[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2021,49(12):11.
APA Shao Wen-Ya.,Liang Yu.,Liu Jian-Xi.,Liu Hong-Tao.,Wang Zhao-Wei.,...&Zhang Yu-Kui.(2021).O-GlcNAcylation Quantification Based on Electron-Transfer/Higher-Energy Collisional Dissociation and Its Application in O-GlcNAcylation Site Mapping of Liver Proteomics in High Fat Diet-fed Mice.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,49(12),11.
MLA Shao Wen-Ya,et al."O-GlcNAcylation Quantification Based on Electron-Transfer/Higher-Energy Collisional Dissociation and Its Application in O-GlcNAcylation Site Mapping of Liver Proteomics in High Fat Diet-fed Mice".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 49.12(2021):11.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Shao Wen-Ya]'s Articles
[Liang Yu]'s Articles
[Liu Jian-Xi]'s Articles
Baidu academic
Similar articles in Baidu academic
[Shao Wen-Ya]'s Articles
[Liang Yu]'s Articles
[Liu Jian-Xi]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Shao Wen-Ya]'s Articles
[Liang Yu]'s Articles
[Liu Jian-Xi]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.