CAS OpenIR
Surface-Engineered Gold Nanoclusters for Stimulated Emission Depletion and Correlated Light and Electron Microscopy Imaging
Yang, Hongwei1,2; Wu, Yayun1; Ruan, Hefei1; Guo, Feng3; Liang, Yuxin1,2; Qin, Gege1,2; Liu, Xiaolong1,2; Zhang, Zhen1; Yuan, Jinghe1; Fang, Xiaohong1,4
2022-02-10
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Pages9
AbstractStimulated emission depletion (STED) nanoscopy is an emerging super-resolution imaging platform for the study of the cellular structure. Developing suitable fluorescent probes of small size, good photostability, and easy functionalization is still in demand. Herein, we introduce a new type of surface-engineered gold nanoclusters (Au NCs) that are ultrasmall (1.7 nm) and ultrabright (QY = 60%) for STED bioimaging. A rigid shell formed by L-arginine (L-Arg) and 6-aza-2thiothymine (ATT) on the Au NC surface enables not only its strong fluorescence in aqueous solution but also its easy chemical modification for specific biomolecule labeling. Au NCs show remarkable performance as STED nanoprobes, including high depletion efficiency, good photobleaching resistance, and low saturation intensity. Super-resolution imaging has been achieved with these Au NCs, and targeted nanoscopic imaging of cellular tubulin has been demonstrated. Moreover, the circular structure of lysosomes in live cells has been revealed. As a Au NC is also an ideal probe for electron microscopy, dual imaging of A beta 42 aggregates with the single labeling probe of Au NCs has been realized in correlative light and electron microscopy (CLEM). This work reports, for the first time, the application of Au NCs as a novel probe in STED and CLEM imaging. With their excellent properties, Au NCs show promising potential for nanoscale bioimaging.
DOI10.1021/acs.analchem.1c03935
Language英语
WOS KeywordSUPERRESOLUTION ; NANOPARTICLES ; FLUORESCENCE ; PROBES
Funding ProjectNational Natural Science Foundation of China[21735006] ; National Natural Science Foundation of China[21890742] ; National Natural Science Foundation of China[91939301] ; National Natural Science Foundation of China[22077124] ; Chinese Academy of Sciences
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
Funding OrganizationNational Natural Science Foundation of China ; Chinese Academy of Sciences
WOS IDWOS:000757849700001
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/51994
Collection中国科学院过程工程研究所
Corresponding AuthorYuan, Jinghe; Fang, Xiaohong
Affiliation1.Chinese Acad Sci, CAS Res Educ Ctr Excellence Mol Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Anal & Testing Ctr, Inst Proc Engn, Beijing 100190, Peoples R China
4.Chinese Acad Sci, Inst Canc & Basic Med, Hangzhou 310022, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Yang, Hongwei,Wu, Yayun,Ruan, Hefei,et al. Surface-Engineered Gold Nanoclusters for Stimulated Emission Depletion and Correlated Light and Electron Microscopy Imaging[J]. ANALYTICAL CHEMISTRY,2022:9.
APA Yang, Hongwei.,Wu, Yayun.,Ruan, Hefei.,Guo, Feng.,Liang, Yuxin.,...&Fang, Xiaohong.(2022).Surface-Engineered Gold Nanoclusters for Stimulated Emission Depletion and Correlated Light and Electron Microscopy Imaging.ANALYTICAL CHEMISTRY,9.
MLA Yang, Hongwei,et al."Surface-Engineered Gold Nanoclusters for Stimulated Emission Depletion and Correlated Light and Electron Microscopy Imaging".ANALYTICAL CHEMISTRY (2022):9.
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