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甘一如; 黄永东; 刘晋生; 张雪竹; 张贵峰
Source Publication化工学报
Other AbstractThymosin α 1 was synthesized manually. The washing condition and the test of the degree of coupling were studied. In addition, the analytical method of crude product was set up. Thymosin α 1 was synthesized with the solid-phase method, by using the base-liable Fmoc group to protect the α-amino acid and N,N′-diisopropylcarbodiimide (DIC) as the coupling regent. The crude product was analyzed by reversed-phase high performance liquid chromatography, SDS-PAGE and liquid chromatography/mass spectroscopy.The synthesis yield was 84.89%. The analytical results indicated that the crude product contained much thymosin α 1, and the purity was 50.3%. DIC solid-phase chemical synthesis of thymosin α 1 was applicable, and the yield was improved greatly compared to other known methods.
Keyword胸腺素α1 固相多肽合成 二异丙基碳二亚胺(Dic) 反相高效液相色谱法 聚丙烯酰胺凝胶电泳(Sdspage)法 液相色谱/质谱(Lc/ms)
Document Type期刊论文
Recommended Citation
GB/T 7714
甘一如,黄永东,刘晋生,等. 胸腺素α_1的DIC固相化学合成与鉴定[J]. 化工学报,2004(02):305-308.
APA 甘一如,黄永东,刘晋生,张雪竹,&张贵峰.(2004).胸腺素α_1的DIC固相化学合成与鉴定.化工学报(02),305-308.
MLA 甘一如,et al."胸腺素α_1的DIC固相化学合成与鉴定".化工学报 .02(2004):305-308.
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