Knowledge Management System Of Institute of process engineering,CAS
| Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo alpha-1,4-Glucanase | |
| Li, Ke1,2,3,4; Li, Xue-qin1,3,4; Li, Guang-xin5; Cui, Lian-jie1,3,4; Qin, Xue-mei1,3,4; Li, Zhen-yu1,3,4; Du, Yu-guang2; Liu, Yue-tao1,3,4; Li, Ai-ping1,3,4; Zhao, Xing-yun1,3,4; Fan, Xin-hui1,3,4 | |
| 2022-02-25 | |
| Source Publication | FRONTIERS IN PHARMACOLOGY
 |
| Volume | 13Pages:15 |
| Abstract | Astragali Radix polysaccharides (APSs) have a wide range of biological activities. Our preliminary experiment showed that APS-II (10 kDa) was the main immunologically active component of APSs. However, the characteristic structure related to activity of APS-II needs further verification and clarification. In this study, APS-II was degraded by endo alpha-1,4-glucosidase. The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column. The structural features of the different products were characterized by HPGPC, monosaccharide composition, Fourier transform infrared spectrum, GC-MS, nuclear magnetic resonance, and UPLC-ESI-QTOF-MS analysis. Specific immune and non-specific immune cell tests were used to identify the most immunogenic fractions of the products. The backbone of P4 was speculated to be alpha-D-1,4-linked glucans and rich in C2 (25.34%) and C6 (34.54%) branches. Immune screening experiments indicated that the activity of P4 was better than that of APS-II and the other three components. In this research, the relationship between the structure of APS-II and the immune activity from the degradation level of polysaccharides was studied, laying a foundation for the quality control and product development of APSs. |
| Keyword | APS-II enzymolysis structure analysis immune activity structure-activity relationship |
| DOI | 10.3389/fphar.2022.839635 |
| Language | 英语 |
| WOS Keyword | ANTIOXIDANT ACTIVITY ; IN-VITRO ; EXTRACTION ; FEATURES |
| Funding Project | National Natural Science Foundation of China[81872962] ; China Postdoctoral Science Foundation Project[2019M650851] ; National Key R&D Program of China[2019YFC1710800] ; Key Projects of Key Research and Development Plan in Shanxi[201603D311101] ; Shanxi Province Technology Innovation Project of Excellent Talent[201605D211030] ; Shanxi Province Technology Innovation Project of Excellent Talent[201705D211020] ; Academic Recovery Research Special Project of Shanxi Agricultural University[2020xshf05] |
| WOS Research Area | Pharmacology & Pharmacy |
| WOS Subject | Pharmacology & Pharmacy |
| Funding Organization | National Natural Science Foundation of China ; China Postdoctoral Science Foundation Project ; National Key R&D Program of China ; Key Projects of Key Research and Development Plan in Shanxi ; Shanxi Province Technology Innovation Project of Excellent Talent ; Academic Recovery Research Special Project of Shanxi Agricultural University |
| WOS ID | WOS:000770703100001 |
| Publisher | FRONTIERS MEDIA SA |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/52442 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Li, Ke; Du, Yu-guang |
| Affiliation | 1.Shanxi Univ, Modern Res Ctr Tradit Chinese Med, Taiyuan, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China 3.Shanxi Univ, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan, Peoples R China 4.Shanxi Univ, Shanxi Key Lab Active Constituents Res & Utiliza, Taiyuan, Peoples R China 5.Shanxi Agr Univ, Coll Agr, Taiyuan, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Ke,Li, Xue-qin,Li, Guang-xin,et al. Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo alpha-1,4-Glucanase[J]. FRONTIERS IN PHARMACOLOGY,2022,13:15. |
| APA | Li, Ke.,Li, Xue-qin.,Li, Guang-xin.,Cui, Lian-jie.,Qin, Xue-mei.,...&Fan, Xin-hui.(2022).Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo alpha-1,4-Glucanase.FRONTIERS IN PHARMACOLOGY,13,15. |
| MLA | Li, Ke,et al."Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo alpha-1,4-Glucanase".FRONTIERS IN PHARMACOLOGY 13(2022):15. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment