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Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation
Wang, Dan1,2; Li, Qiang1,2; Yang, Maohua1,2; Zhang, Yunjian3; Su, Zhiguo1; Xing, Jianmin1; Xing, JM
2011
Source PublicationPROCESS BIOCHEMISTRY
ISSN1359-5113
Volume46Issue:1Pages:365-371
AbstractSuccinic acid is considered to be one of the key platform chemicals used in a variety of industrial applications. The exploitation of biomass to produce succinic acid requires a microbial type that can ferment the mixture of reducing sugars derived from lignocellulose. The recombinant Escherichia coli strains with homologous or cyanobacterial ppc overexpression and IdhA, pflB, ptsG mutations were constructed, and the mixed sugar fermentations were carried out with the prominent strain SD121. Then, a modeled corn stalk hydrolysates containing 30 g l(-1) glucose, 10 g l(-1) xylose and 2.5 g l(-1) arabinose was applied for succinic acid fermentation with SD121. A yield of 0.77 g succinic acid g(-1) total sugar was achieved. Fermentation of corn stalk hydrolysates with SD121 produced a final succinic acid concentration of 36.55 g l(-1) with a higher yield of 0.83 g g(-1) total sugar in anaerobic bottles. In two-stage fermentation process in bioreactor, initial aerobic growth facilitated the subsequent anaerobic succinic acid production with a final concentration of 57.81 g l(-1), and a yield of 0.87 g g(-1), total sugar. This was the first report of succinic acid production from corn stalk hydrolysates by metabolically engineered Escherichia coli. The higher succinic acid yield from corn stalk hydrolysates compared to modeled sugar mixtures, showed a great potential usage of renewable biomass as a feedstock for an economical succinic acid production using E. coli. (C) 2010 Elsevier Ltd. All rights reserved.
KeywordGlucose Phosphotransferase Escherichia Coli Succinic Acid Phosphoenolpyruvate Carboxylase Corn Stalk Hydrolysates
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.procbio.2010.09.012
Indexed BySCI
Language英语
WOS KeywordCATABOLITE REPRESSION MUTANTS ; PHOSPHOENOLPYRUVATE CARBOXYLASE ; ACTINOBACILLUS-SUCCINOGENES ; LACTATE-DEHYDROGENASE ; PYRUVATE-CARBOXYLASE ; SUGAR MIXTURES ; MALIC ENZYME ; FERMENTATION ; EXPRESSION ; OVEREXPRESSION
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000286538300051
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Cited Times:42[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5253
Collection生化工程国家重点实验室
Corresponding AuthorXing, JM
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.China Univ Min & Technol Beijing, Dept Bioengn, Sch Chem & Environm Engn, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Wang, Dan,Li, Qiang,Yang, Maohua,et al. Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation[J]. PROCESS BIOCHEMISTRY,2011,46(1):365-371.
APA Wang, Dan.,Li, Qiang.,Yang, Maohua.,Zhang, Yunjian.,Su, Zhiguo.,...&Xing, JM.(2011).Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation.PROCESS BIOCHEMISTRY,46(1),365-371.
MLA Wang, Dan,et al."Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation".PROCESS BIOCHEMISTRY 46.1(2011):365-371.
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