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Enzyme adsorption and recycling during hydrolysis of wheat straw lignocellulose
Qi, Benkun; Chen, Xiangrong; Su, Yi; Wan, Yinhua; Wan, YH
2011-02-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume102Issue:3Pages:2881-2889
AbstractThe objective of this research was to investigate cellulase adsorption and recycling during enzymatic hydrolysis of two differently pretreated wheat straws (WS). Dilute acid treated WS showed lower hydrolysis yield of polysaccharides fraction and adsorbed more cellulase with hydrolyzed residue than dilute alkali treated sample. Four methods capable of recovering and recycling the enzyme bound to the residual substrate and the enzyme free in solution were used for three consecutive rounds of hydrolysis to compare their recycling efficiencies. Compared to the absorption recycling method, ultrafiltration recycling method possessed the capacity to retain p-glucosidase, thereby avoiding the supplementation of fresh p-glucosidase in subsequent rounds of hydrolysis. It was found that whatever recycling method was used, better recycling results were obtained for dilute alkali treated substrate than for dilute acid treated substrate. These results suggested that the great difference in the lignin content between acid treated WS and alkali treated WS would significantly affect enzymatic hydrolysis, cellulase adsorption and cellulase recycling efficiencies. (C) 2010 Elsevier Ltd. All rights reserved.
KeywordCellulase Adsorption Ultrafiltration Enzyme Recycling Enzyme Recovery
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.biortech.2010.10.092
Indexed BySCI
Language英语
WOS KeywordCELLULASE ADSORPTION ; CORN STOVER ; RECOVERY ; SOFTWOOD ; LIGNIN ; OPTIMIZATION ; COMPLEX ; ETHANOL ; BIOMASS ; SACCHARIFICATION
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000286904500104
Citation statistics
Cited Times:101[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5262
Collection生化工程国家重点实验室
Corresponding AuthorWan, YH
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Qi, Benkun,Chen, Xiangrong,Su, Yi,et al. Enzyme adsorption and recycling during hydrolysis of wheat straw lignocellulose[J]. BIORESOURCE TECHNOLOGY,2011,102(3):2881-2889.
APA Qi, Benkun,Chen, Xiangrong,Su, Yi,Wan, Yinhua,&Wan, YH.(2011).Enzyme adsorption and recycling during hydrolysis of wheat straw lignocellulose.BIORESOURCE TECHNOLOGY,102(3),2881-2889.
MLA Qi, Benkun,et al."Enzyme adsorption and recycling during hydrolysis of wheat straw lignocellulose".BIORESOURCE TECHNOLOGY 102.3(2011):2881-2889.
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