| Enzyme adsorption and recycling during hydrolysis of wheat straw lignocellulose | |
| Qi, Benkun; Chen, Xiangrong; Su, Yi; Wan, Yinhua; Wan, YH | |
| 2011-02-01 | |
| Source Publication | BIORESOURCE TECHNOLOGY
 |
| ISSN | 0960-8524 |
| Volume | 102Issue:3Pages:2881-2889 |
| Abstract | The objective of this research was to investigate cellulase adsorption and recycling during enzymatic hydrolysis of two differently pretreated wheat straws (WS). Dilute acid treated WS showed lower hydrolysis yield of polysaccharides fraction and adsorbed more cellulase with hydrolyzed residue than dilute alkali treated sample. Four methods capable of recovering and recycling the enzyme bound to the residual substrate and the enzyme free in solution were used for three consecutive rounds of hydrolysis to compare their recycling efficiencies. Compared to the absorption recycling method, ultrafiltration recycling method possessed the capacity to retain p-glucosidase, thereby avoiding the supplementation of fresh p-glucosidase in subsequent rounds of hydrolysis. It was found that whatever recycling method was used, better recycling results were obtained for dilute alkali treated substrate than for dilute acid treated substrate. These results suggested that the great difference in the lignin content between acid treated WS and alkali treated WS would significantly affect enzymatic hydrolysis, cellulase adsorption and cellulase recycling efficiencies. (C) 2010 Elsevier Ltd. All rights reserved. |
| Keyword | Cellulase Adsorption Ultrafiltration Enzyme Recycling Enzyme Recovery |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| DOI | 10.1016/j.biortech.2010.10.092 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | CELLULASE ADSORPTION ; CORN STOVER ; RECOVERY ; SOFTWOOD ; LIGNIN ; OPTIMIZATION ; COMPLEX ; ETHANOL ; BIOMASS ; SACCHARIFICATION |
| WOS Research Area | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS Subject | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS ID | WOS:000286904500104 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/5262 |
| Collection | 生化工程国家重点实验室 |
| Corresponding Author | Wan, YH |
| Affiliation | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Qi, Benkun,Chen, Xiangrong,Su, Yi,et al. Enzyme adsorption and recycling during hydrolysis of wheat straw lignocellulose[J]. BIORESOURCE TECHNOLOGY,2011,102(3):2881-2889. |
| APA | Qi, Benkun,Chen, Xiangrong,Su, Yi,Wan, Yinhua,&Wan, YH.(2011).Enzyme adsorption and recycling during hydrolysis of wheat straw lignocellulose.BIORESOURCE TECHNOLOGY,102(3),2881-2889. |
| MLA | Qi, Benkun,et al."Enzyme adsorption and recycling during hydrolysis of wheat straw lignocellulose".BIORESOURCE TECHNOLOGY 102.3(2011):2881-2889. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 20131108-1033-复件 1-s（1945KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | Application Full Text | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment