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| Biooxidation of ferrous iron by immobilized Acidithiobacillus ferrooxidans in poly(vinyl alcohol) cryogel carriers | |
| Alternative Title | Biotechnol. Lett. |
| Long, ZE; Huang, YH; Cai, ZL; Cong, W; Fan, OY | |
| 2003-02-01 | |
| Source Publication | BIOTECHNOLOGY LETTERS
 |
| ISSN | 0141-5492 |
| Volume | 25Issue:3Pages:245-249 |
| Abstract | PVA-cryogels entrapping about 10 9 cells of Acidithiobacillus ferrooxidans per ml of gel were prepared by freezing-thawing procedure, and the biooxidation of Fe2+ by immobilized cells was investigated in a 0.365 l packed-bed bioreactor. Fe2+ oxidation fits a plug-flow reaction model well. A maximum oxidation rate of 3.1 g Fe2+ l(-1) h(-1) was achieved at the dilution rate of 0.4 h(-1) or higher, while no obvious precipitate was determined at this time. In addition, cell-immobilized PVA-cryogels packed in bioreactor maintained their oxidative ability for more than two months under non-sterile conditions. Nomenclature: C-A0 - Concentration of Fe2+ in feed stream (g l(-1)) C-A - Concentration of Fe2+ in outlet stream (g l(-1)) D - Dilution rate of the packed-bed bioreactor (h(-1)) F - Volumetric flow rate of iron solution (l h(-1)) F-A0 - Mass flowrate of Fe2+ in the feed stream (g h(-1)) K - Kinetic constant (11(-1) h(-)1) r(A) - Oxidation rate of Fe2+ (g l(-1) h(-1)) V - Volume of packed-bed bioreactor (l) X-A - Conversion ratio of Fe2+ (%); PVA-cryogels entrapping about 10 9 cells of Acidithiobacillus ferrooxidans per ml of gel were prepared by freezing-thawing procedure, and the biooxidation of Fe2+ by immobilized cells was investigated in a 0.365 l packed-bed bioreactor. Fe2+ oxidation fits a plug-flow reaction model well. A maximum oxidation rate of 3.1 g Fe2+ l(-1) h(-1) was achieved at the dilution rate of 0.4 h(-1) or higher, while no obvious precipitate was determined at this time. In addition, cell-immobilized PVA-cryogels packed in bioreactor maintained their oxidative ability for more than two months under non-sterile conditions. |
| Keyword | Acidithiobacillus Ferrooxidans Biooxidation Of Ferrous Iron Immobilization Packed-bed Bioreactor Poly(Vinyl Alcohol) Cryogel |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | PACKED-BED REACTORS ; THIOBACILLUS-FERROOXIDANS ; SULFATE OXIDATION ; KINETICS ; CELLS |
| WOS Research Area | Biotechnology & Applied Microbiology |
| WOS Subject | Biotechnology & Applied Microbiology |
| WOS ID | WOS:000180831100011 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/5270 |
| Collection | 研究所（批量导入） |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Jiangxi Normal Univ, Coll Life Sci, Nanchang 330027, Peoples R China |
| Recommended Citation GB/T 7714 | Long, ZE,Huang, YH,Cai, ZL,et al. Biooxidation of ferrous iron by immobilized Acidithiobacillus ferrooxidans in poly(vinyl alcohol) cryogel carriers[J]. BIOTECHNOLOGY LETTERS,2003,25(3):245-249. |
| APA | Long, ZE,Huang, YH,Cai, ZL,Cong, W,&Fan, OY.(2003).Biooxidation of ferrous iron by immobilized Acidithiobacillus ferrooxidans in poly(vinyl alcohol) cryogel carriers.BIOTECHNOLOGY LETTERS,25(3),245-249. |
| MLA | Long, ZE,et al."Biooxidation of ferrous iron by immobilized Acidithiobacillus ferrooxidans in poly(vinyl alcohol) cryogel carriers".BIOTECHNOLOGY LETTERS 25.3(2003):245-249. |
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| Biooxidation of ferr（76KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
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