CAS OpenIR
Alicyclobacillus curvatus sp. nov. and Alicyclobacillus mengziensis sp. nov., two acidophilic bacteria isolated from acid mine drainage
Jiang, Zhen1,2; Wu, Dildar1,3; Liang, Zong-Lin1,2; Li, Xiu-Tong1,2; Huang, Ye1,2; Zhou, Nan1; Liu, Zheng-Hua4; Zhang, Guang-Ji5,6; Jia, Yan5,6; Yin, Hua-Qun4; Liu, Shuang-Jiang1,2,5; Jiang, Cheng-Ying1,2,5
2022
Source PublicationINTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
ISSN1466-5026
Volume72Issue:3Pages:8
AbstractTwo acidophilic strains, designated as ALEF1(T) and S30H14(T), were isolated from acid mine drainage sediment. Cells of both strains were Gram-stain-positive, aerobic, endospore-forming rods. Strains ALEF1(T) and S30H14(T) were acidophilic and mesophilic, the former grew at 20-40 degrees C (optimum, 30 degrees C) and pH 2.5-4.5 (optimum, pH 3.5), while the latter grew at 20-45 degrees C (optimum, 30 degrees C) and pH 2.0-5.5 (optimum, pH 4.5). The 16S rRNA gene-based sequence analysis revealed that strains ALEF1(T) and S30H14(T) belonged to the genus Alicyclobacillus, and were phylogenetically close to Alicyclobacillus ferrooxydans TC-34(T) with 97.1 and 97.4% similarity, respectively. The similarity between the two novel strains was 98.6 %. The average nucleotide identity value between the genome sequences of ALEF1(T) and S30H14(T) was 79.5 %, and that between each of the two isolates and A. ferrooxydans TC-34(T) were 72.0 and 74.3 %. In addition, the digital DNA-DNA hybridization value between ALEF1(T) and S30H14(T) was 24.9 %, between strain ALEF1(T) and A. ferrooxydans TC-34(T) was 21.7 %, and between S30H14(T) and A. ferrooxydans TC-34(T) was 26.3 %, far below the interspecies threshold. Both strains could utilize diverse carbon sources for heterotrophic growth; strain ALEF1(T) could utilize ferrous iron as the energy source for autotrophic growth. Menaquinone 7 was the only quinone detected in either strain. Both strains contained anteiso-C-15:0 and anteiso-C-17:0, while omega-alicyclic fatty acids were not detected. Based on their phylogenetic positions, as well as phenotypic and genomic data, it is considered that strains ALEF1(T) and S30H14(T) represent two novel species within the genus Alicyclobacillus, for which the names Alicyclobacillus curvatus sp. nov. (type strain ALEF1(T)=CGMCC 1.17055(T)=KCTC 43124(T)) and Alicyclobacillus mengziensis sp. nov. (S30H14(T)=CGMCC 1.17050(T)=KCTC 43125(T)) are proposed.
KeywordAlicyclobacillus curvatus Alicyclobacillus mengziensis acid mine drainage acidophile
DOI10.1099/ijsem.0.005285
Language英语
WOS KeywordBACILLUS-ACIDOCALDARIUS ; OXIDIZING BACTERIUM ; EMENDED DESCRIPTION ; FATTY-ACIDS ; GENUS ; EXTRACTION
Funding ProjectMajor Research Plan of the National Natural Science Foundation of China[91851206] ; CAS Engineering Laboratory for Advanced Microbial Technology of Agriculture, Chinese Academy of Sciences[KFJ-PTXM-016] ; CAS-NSTDA Joint Research Program[53211KYSB20200039] ; Innovation Academy for Green Manufacture, Chinese Academy of Sciences[IAGM2020C24] ; National Natural Science Foundation of China[31670124]
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
Funding OrganizationMajor Research Plan of the National Natural Science Foundation of China ; CAS Engineering Laboratory for Advanced Microbial Technology of Agriculture, Chinese Academy of Sciences ; CAS-NSTDA Joint Research Program ; Innovation Academy for Green Manufacture, Chinese Academy of Sciences ; National Natural Science Foundation of China
WOS IDWOS:000785355900010
PublisherMICROBIOLOGY SOC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/52882
Collection中国科学院过程工程研究所
Corresponding AuthorLiu, Shuang-Jiang; Jiang, Cheng-Ying
Affiliation1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Kashi Univ, Coll Life & Geog Sci, Kashi 844000, Peoples R China
4.Cent South Univ, Sch Minerals Proc & Bioengn, Key Lab Biomet, Minist Educ, Changsha, Peoples R China
5.Chinese Acad Sci, Innovat Acad Green Manufacture, Beijing 100190, Peoples R China
6.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Zhen,Wu, Dildar,Liang, Zong-Lin,et al. Alicyclobacillus curvatus sp. nov. and Alicyclobacillus mengziensis sp. nov., two acidophilic bacteria isolated from acid mine drainage[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2022,72(3):8.
APA Jiang, Zhen.,Wu, Dildar.,Liang, Zong-Lin.,Li, Xiu-Tong.,Huang, Ye.,...&Jiang, Cheng-Ying.(2022).Alicyclobacillus curvatus sp. nov. and Alicyclobacillus mengziensis sp. nov., two acidophilic bacteria isolated from acid mine drainage.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,72(3),8.
MLA Jiang, Zhen,et al."Alicyclobacillus curvatus sp. nov. and Alicyclobacillus mengziensis sp. nov., two acidophilic bacteria isolated from acid mine drainage".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 72.3(2022):8.
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