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From sucrose to fructo-oligosaccharides: Production and purification of fructo-oligosaccharides by an integrated enzymatic catalysis and membrane separation process

Cao, Weilei1,2; Deng, Tingting3; Cao, Weifeng1,2; Shen, Fei1,2; Wan, Yinhua1,2
2022-05-01
Source PublicationSEPARATION AND PURIFICATION TECHNOLOGY
ISSN1383-5866
Volume288Pages:11
AbstractFructo-oligosaccharides (FOS) are a group of linear fructose oligomers with numerous health benefits, which can be synthesized by the transfructosylation of sucrose using fructosyltransferases (FTases). In this study, it was found that the selected FTases showed both hydrolytic and transfructosylating activities. For FOS production, the hydrolytic activities of FTases became significant when the reaction time was above 1 h. Thus, FTases needed to be inactivated or removed from the reactor after achieving the highest FOS conversion. We therefore developed an integrated ultrafiltration-diafiltration-concentration process for FTases reuse and FOS purification. Because of the 100% rejection of FTases activity and a higher average flux, a PES-10 ultrafiltration membrane was selected for FTases recovery, where the cake layer formation was the main membrane fouling mechanism. Then, a fed batch reaction process was developed for FOS production, where the hydrolytic behavior of FTases was negligible, and the FTases dosage was reduced by 75% compared to single batch operation (four batches). Moreover, the FOS production was comparable, and 95.5% FTases was recovered by the PES10 membrane after the four fed-batches processes. Subsequently, a NF5 nanofiltration membrane was selected to further separate FOS from the FTases-free FOS solution by a constant volume diafiltration process under 45 ?, and the monosaccharides in the reaction mixture (glucose and fructose) were completely removed, increasing the FOS purity from 55.8% to 92.3%. Finally, the FOS and sucrose in the diafiltration permeate was recovered by a DL nanofiltration membrane under 60 C. The outcome of this work offers a complete technical route for FOS production using sucrose as substrate.
KeywordFructo-oligosaccharides Membrane Fructosyltransferases Diafiltration
DOI10.1016/j.seppur.2022.120678
Language英语
WOS KeywordFRUCTOSYLTRANSFERASE ; IMMOBILIZATION ; FERMENTATION ; COCULTURE
Funding ProjectBeijing Natural Science Foundation, China[5182025] ; National Natural Science Foundation of China, China[21406240] ; National High Technology Research and Development Program of China[2015AA021002] ; National High Technology Research and Development Program of China[2014AA021005] ; Science and Technology Service Network Program of Chinese Academy of Sciences
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
Funding OrganizationBeijing Natural Science Foundation, China ; National Natural Science Foundation of China, China ; National High Technology Research and Development Program of China ; Science and Technology Service Network Program of Chinese Academy of Sciences
WOS IDWOS:000782997100005
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/52892
Collection中国科学院过程工程研究所
Corresponding AuthorCao, Weifeng; Wan, Yinhua
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China
3.Chinese Acad Inspect & Quarantine, Agroprod Safety Res Ctr, Beijing 100123, Peoples R China
Recommended Citation
GB/T 7714
Cao, Weilei,Deng, Tingting,Cao, Weifeng,et al.

From sucrose to fructo-oligosaccharides: Production and purification of fructo-oligosaccharides by an integrated enzymatic catalysis and membrane separation process

[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2022,288:11.
APA Cao, Weilei,Deng, Tingting,Cao, Weifeng,Shen, Fei,&Wan, Yinhua.(2022).

From sucrose to fructo-oligosaccharides: Production and purification of fructo-oligosaccharides by an integrated enzymatic catalysis and membrane separation process

.SEPARATION AND PURIFICATION TECHNOLOGY,288,11.
MLA Cao, Weilei,et al."

From sucrose to fructo-oligosaccharides: Production and purification of fructo-oligosaccharides by an integrated enzymatic catalysis and membrane separation process

".SEPARATION AND PURIFICATION TECHNOLOGY 288(2022):11.
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