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| Development of HPV58 type-specific antibodies and detection kit | |
| Liu, Yuying1,2,3; Zhang, Yao1; Zhang, Guifeng2; Yang, Zengmin1; Wang, Yan1; Wu, Shuming1; Chen, Dan1; Zhang, Haijiang1; Liu, Yongjiang1 | |
| 2022-04-22 | |
| Source Publication | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
 |
| ISSN | 1082-6068 |
| Pages | 7 |
| Abstract | Currently, human papillomavirus (HPV) vaccines are in short supply, so the development of HPV vaccines has a broad market prospect. The 3-, 9-, and 15-valent HPV vaccines developed by ourselves all contain HPV58-derived antigen components. It is important to detect HPV 58 during vaccine production. Here, we introduced a development process of HPV58 type-specific antibodies and a detection kit. Briefly, HPV58 L1-Virus Like Particles (VLPs) were used as antigens to immunize mice, followed by extraction of the ascites to prepare hybridoma cells. After culturing, the supernatants containing secreted antibodies were harvested, purified, and screened to obtain monoclonal antibodies (mAbs). In the pool of attained monoclonal antibodies, we selected 2F7 and 2G7 to evaluate their subtypes, specificity, neutralizing activity, serum competition, binding affinity and gene sequencing. Finally, an enzyme-linked immunosorbent assay (ELISA) detection kit was assembled with 2F7 and 2G7 mAbs which possessed high specificity to HPV58 L1-VLPs. The detection kit developed by 2F7 and 2G7 could be adopted to specifically detect HPV58 L1 protein with good linearity and detection range, which could be widely used in clinical testing and quality control in the production of HPV vaccines. |
| Keyword | Detection kit ELISA HPV58 monoclonal antibody vaccine |
| DOI | 10.1080/10826068.2022.2065686 |
| Language | 英语 |
| WOS Keyword | COMPARATIVE MODELING ANALYSIS ; CERVICAL-CANCER ELIMINATION ; 78 LOW-INCOME ; PAPILLOMAVIRUS ; VACCINATION ; INFECTION ; IMPACT ; ASSAY |
| WOS Research Area | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS Subject | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS ID | WOS:000786420900001 |
| Publisher | TAYLOR & FRANCIS INC |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/52900 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Zhang, Haijiang; Liu, Yongjiang |
| Affiliation | 1.Beijing Hlth Guard Biotechnol Inc, BDA, Unit 201,Block 2,Longsheng Ind Pk, Beijing 100176, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Sch Chem & Engn, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Liu, Yuying,Zhang, Yao,Zhang, Guifeng,et al. Development of HPV58 type-specific antibodies and detection kit[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2022:7. |
| APA | Liu, Yuying.,Zhang, Yao.,Zhang, Guifeng.,Yang, Zengmin.,Wang, Yan.,...&Liu, Yongjiang.(2022).Development of HPV58 type-specific antibodies and detection kit.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,7. |
| MLA | Liu, Yuying,et al."Development of HPV58 type-specific antibodies and detection kit".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY (2022):7. |
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