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Magnet-assisted electrochemical immunosensor based on surface-clean Pd-Au nanosheets for sensitive detection of SARS-CoV-2 spike protein

Zhao, Jialin1,2; Zhao, Fu1; Li, Haolin1,2; Xiong, Youlin1; Cai, Shuangfei1; Wang, Chen1; Chen, Yunfa3; Han, Ning3; Yang, Rong1,2
2022-02-01
Source PublicationELECTROCHIMICA ACTA
ISSN0013-4686
Volume404Pages:9
AbstractTracking and monitoring of low concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can effectively control asymptomatic transmission of current coronavirus disease 2019 (COVID-19) in the early stages of infection. Here, we highlight an electrochemical immunosensor for sensitive detection of SARS-CoV-2 antigen marker spike protein. The surface-clean Pd-Au nanosheets as a substrate for efficient sensing and signal output have been synthesized. The morphology, chemical states and excellent stable electrochemical properties of this surface-clean heterostructures have been studied. Functionalized superparamagnetic nanoparticles (MNPs) were introduced as sample separators and signal amplifiers. This biosensor was tested in phosphate buffered saline (PBS) and nasopharyngeal samples. The results showed that the sensor has a wide linear dynamic range (0.01 ng mL(-1) to 1000 ng mL(-1)) with a low detection limit (0.0072 ng mL(-1)), which achieved stable and sensitive detection of the spike protein. Therefore, this immunosensing method provides a promising electrochemical measurement tool, which can furnish ideas for early screening and the reasonable optimization of detection methods of SARS-CoV-2. (C)& nbsp;2021 Elsevier Ltd. All rights reserved.
KeywordSARS-CoV-2 spike protein Pd-Au nanosheets Differential pulse voltammetry Immunosensor
DOI10.1016/j.electacta.2021.139766
Language英语
WOS KeywordNANOCOMPOSITES
Funding ProjectStrategic Priority Research Pro-gram of the Chinese Academy of Sciences[XDB36000000] ; National key research and development program from the Ministry of Science and Technology of China[2021YFE0112600] ; National key research and development program from the Ministry of Science and Technology of China[2016YFC0207102]
WOS Research AreaElectrochemistry
WOS SubjectElectrochemistry
Funding OrganizationStrategic Priority Research Pro-gram of the Chinese Academy of Sciences ; National key research and development program from the Ministry of Science and Technology of China
WOS IDWOS:000779416300002
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/52970
Collection中国科学院过程工程研究所
Corresponding AuthorYang, Rong
Affiliation1.Univ Chinese Acad Sci, Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci,Ctr Mat Sci & Optoelec, CAS Key Lab Biomed Effects Nanomat & Nanosafety, 11 ZhongGuanCun BeiYiTiao, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Sino Danish Coll, Sino Danish Ctr Educ & Res, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Multiphase Complex Syst, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Jialin,Zhao, Fu,Li, Haolin,et al.

Magnet-assisted electrochemical immunosensor based on surface-clean Pd-Au nanosheets for sensitive detection of SARS-CoV-2 spike protein

[J]. ELECTROCHIMICA ACTA,2022,404:9.
APA Zhao, Jialin.,Zhao, Fu.,Li, Haolin.,Xiong, Youlin.,Cai, Shuangfei.,...&Yang, Rong.(2022).

Magnet-assisted electrochemical immunosensor based on surface-clean Pd-Au nanosheets for sensitive detection of SARS-CoV-2 spike protein

.ELECTROCHIMICA ACTA,404,9.
MLA Zhao, Jialin,et al."

Magnet-assisted electrochemical immunosensor based on surface-clean Pd-Au nanosheets for sensitive detection of SARS-CoV-2 spike protein

".ELECTROCHIMICA ACTA 404(2022):9.
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