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Inhibition of aggregation by media selection, sample loading and elution in size exclusion chromatographic refolding of denatured bovine carbonic anhydrase B
Alternative TitleJ. Biochem. Biophys. Methods
Gu, ZY; Zhu, XN; Ni, SW; Zhou, HM; Su, ZG
2003-06-30
Source PublicationJOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
ISSN0165-022X
Volume56Issue:1-3Pages:165-175
AbstractThe effects of gel media, sample application and elution flowrate on the activity recovery and aggregation in refolding of bovine carbonic anhydrase B (CAB) by size exclusion chromatography (SEC) were investigated. Variation in aggregation was demonstrated visibly by the comparison of refolding profiles under different operating conditions. Some principles with regard to practical application were proposed. Meanwhile, the analysis of relationship between peak resolution and activity recovery provided evidences for the mechanism of size exclusion chromatography protein refolding. (C) 2003 Published by Elsevier Science B.V.; The effects of gel media, sample application and elution flowrate on the activity recovery and aggregation in refolding of bovine carbonic anhydrase B (CAB) by size exclusion chromatography (SEC) were investigated. Variation in aggregation was demonstrated visibly by the comparison of refolding profiles under different operating conditions. Some principles with regard to practical application were proposed. Meanwhile, the analysis of relationship between peak resolution and activity recovery provided evidences for the mechanism of size exclusion chromatography protein refolding. (C) 2003 Published by Elsevier Science B.V.
KeywordAggregation Refolding Size Exclusion Chromatography Resolution Bovine Carbonic Anhydrase b
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/S0165-022X(03)00057-5
URL查看原文
Indexed BySCI
Language英语
WOS KeywordINCLUSION-BODIES ; PROTEIN ; PURIFICATION ; REACTIVATION
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000184297300011
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5303
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
Recommended Citation
GB/T 7714
Gu, ZY,Zhu, XN,Ni, SW,et al. Inhibition of aggregation by media selection, sample loading and elution in size exclusion chromatographic refolding of denatured bovine carbonic anhydrase B[J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS,2003,56(1-3):165-175.
APA Gu, ZY,Zhu, XN,Ni, SW,Zhou, HM,&Su, ZG.(2003).Inhibition of aggregation by media selection, sample loading and elution in size exclusion chromatographic refolding of denatured bovine carbonic anhydrase B.JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS,56(1-3),165-175.
MLA Gu, ZY,et al."Inhibition of aggregation by media selection, sample loading and elution in size exclusion chromatographic refolding of denatured bovine carbonic anhydrase B".JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS 56.1-3(2003):165-175.
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