Knowledge Management System Of Institute of process engineering,CAS
| Inhibition of aggregation by media selection, sample loading and elution in size exclusion chromatographic refolding of denatured bovine carbonic anhydrase B | |
| Alternative Title | J. Biochem. Biophys. Methods |
| Gu, ZY; Zhu, XN; Ni, SW; Zhou, HM; Su, ZG | |
| 2003-06-30 | |
| Source Publication | JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
 |
| ISSN | 0165-022X |
| Volume | 56Issue:1-3Pages:165-175 |
| Abstract | The effects of gel media, sample application and elution flowrate on the activity recovery and aggregation in refolding of bovine carbonic anhydrase B (CAB) by size exclusion chromatography (SEC) were investigated. Variation in aggregation was demonstrated visibly by the comparison of refolding profiles under different operating conditions. Some principles with regard to practical application were proposed. Meanwhile, the analysis of relationship between peak resolution and activity recovery provided evidences for the mechanism of size exclusion chromatography protein refolding. (C) 2003 Published by Elsevier Science B.V.; The effects of gel media, sample application and elution flowrate on the activity recovery and aggregation in refolding of bovine carbonic anhydrase B (CAB) by size exclusion chromatography (SEC) were investigated. Variation in aggregation was demonstrated visibly by the comparison of refolding profiles under different operating conditions. Some principles with regard to practical application were proposed. Meanwhile, the analysis of relationship between peak resolution and activity recovery provided evidences for the mechanism of size exclusion chromatography protein refolding. (C) 2003 Published by Elsevier Science B.V. |
| Keyword | Aggregation Refolding Size Exclusion Chromatography Resolution Bovine Carbonic Anhydrase b |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1016/S0165-022X(03)00057-5 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | INCLUSION-BODIES ; PROTEIN ; PURIFICATION ; REACTIVATION |
| WOS Research Area | Biochemistry & Molecular Biology ; Biophysics |
| WOS Subject | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics |
| WOS ID | WOS:000184297300011 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/5303 |
| Collection | 研究所（批量导入） |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China |
| Recommended Citation GB/T 7714 | Gu, ZY,Zhu, XN,Ni, SW,et al. Inhibition of aggregation by media selection, sample loading and elution in size exclusion chromatographic refolding of denatured bovine carbonic anhydrase B[J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS,2003,56(1-3):165-175. |
| APA | Gu, ZY,Zhu, XN,Ni, SW,Zhou, HM,&Su, ZG.(2003).Inhibition of aggregation by media selection, sample loading and elution in size exclusion chromatographic refolding of denatured bovine carbonic anhydrase B.JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS,56(1-3),165-175. |
| MLA | Gu, ZY,et al."Inhibition of aggregation by media selection, sample loading and elution in size exclusion chromatographic refolding of denatured bovine carbonic anhydrase B".JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS 56.1-3(2003):165-175. |
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| File Name/Size | DocType | Version | Access | License | ||
| Inhibition of aggreg（190KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
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