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Optimization of cryopreservation of Artemisia annua L. callus
Alternative TitleBiotechnol. Lett.
An, CS; Wang, XD; Yuan, XF; Zhao, B; Wang, YC
2003
Source PublicationBIOTECHNOLOGY LETTERS
ISSN0141-5492
Volume25Issue:1Pages:35-38
AbstractCryopreservation of callus tissue of Artimisia annua L. was optimized. Two lines of calli were precultured on MS medium with 5% (v/v) dimethyl sulfoxide, and protected by a cryoprotectant containing 15% (v/v) ethylene glycol, 15% (v/v) dimethyl sulfoxide, 30% (v/v) glycerol and 13.6% (w/v) sucrose. The highest survival rate of callus A201 reached 87% after it was pretreated at 25degreesC, cryopreserved by liquid nitrogen, recovered in water bath at 25degreesC and reloaded at 25degreesC with 34% (w/v) sucrose solution, and that of callus A202 reached 78% after it was treated as callus A201, except pretreated at 35degreesC, recovered at 35degreesC and reloaded with 47.8% (w/v) sucrose solution.; Cryopreservation of callus tissue of Artimisia annua L. was optimized. Two lines of calli were precultured on MS medium with 5% (v/v) dimethyl sulfoxide, and protected by a cryoprotectant containing 15% (v/v) ethylene glycol, 15% (v/v) dimethyl sulfoxide, 30% (v/v) glycerol and 13.6% (w/v) sucrose. The highest survival rate of callus A201 reached 87% after it was pretreated at 25degreesC, cryopreserved by liquid nitrogen, recovered in water bath at 25degreesC and reloaded at 25degreesC with 34% (w/v) sucrose solution, and that of callus A202 reached 78% after it was treated as callus A201, except pretreated at 35degreesC, recovered at 35degreesC and reloaded with 47.8% (w/v) sucrose solution.
KeywordArtemisinin Artemisia Annua L. Callus Cryopreservation Optimization
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000180046100007
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5319
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
An, CS,Wang, XD,Yuan, XF,et al. Optimization of cryopreservation of Artemisia annua L. callus[J]. BIOTECHNOLOGY LETTERS,2003,25(1):35-38.
APA An, CS,Wang, XD,Yuan, XF,Zhao, B,&Wang, YC.(2003).Optimization of cryopreservation of Artemisia annua L. callus.BIOTECHNOLOGY LETTERS,25(1),35-38.
MLA An, CS,et al."Optimization of cryopreservation of Artemisia annua L. callus".BIOTECHNOLOGY LETTERS 25.1(2003):35-38.
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