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Preparation and characterization of dimeric bovine hemoglobin tetramers
Alternative TitleJ. Protein Chem.
Hu, T; Li, DX; Su, ZG
2003-07-01
Source PublicationJOURNAL OF PROTEIN CHEMISTRY
ISSN0277-8033
Volume22Issue:5Pages:411-416
AbstractDimeric bovine hemoglobin (Hb) tetramers were prepared by a one-step solid phase adsorption method. Briefly, Hb was absorbed by the solid phase, Q Sepharose Fast Flow media, followed by reaction with the glutaraldehyde and elution procedure. Then, dimeric bovine Hb tetramers were formed and purified from Hb tetramers by anion-exchange chromatography based on Protein-Pak DEAE 8HR. The dimeric Hb tetramer showed a P-50 value of 15.9 mm Hg, oxygen transporting efficiency of 14.2%, and Hill coefficient of 1.72. The number of Bohr protons released for dimeric Hb tetramers was 0.59 H/tetramer, which was 39% of that of native bovine Hb. The number of chloride ions released on oxygenation was 0.60/ tetramer for dimeric Hb tetramers, which was 46% of that of native bovine Hb.; Dimeric bovine hemoglobin (Hb) tetramers were prepared by a one-step solid phase adsorption method. Briefly, Hb was absorbed by the solid phase, Q Sepharose Fast Flow media, followed by reaction with the glutaraldehyde and elution procedure. Then, dimeric bovine Hb tetramers were formed and purified from Hb tetramers by anion-exchange chromatography based on Protein-Pak DEAE 8HR. The dimeric Hb tetramer showed a P-50 value of 15.9 mm Hg, oxygen transporting efficiency of 14.2%, and Hill coefficient of 1.72. The number of Bohr protons released for dimeric Hb tetramers was 0.59 H/tetramer, which was 39% of that of native bovine Hb. The number of chloride ions released on oxygenation was 0.60/ tetramer for dimeric Hb tetramers, which was 46% of that of native bovine Hb.
KeywordDimeric Hemoglobin Tetramers Hemoglobin Glutaraldehyde Oxygen Therapeutics
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordSTROMA-FREE HEMOGLOBIN ; GLUTARALDEHYDE
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000186865700002
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Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5327
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Hu, T,Li, DX,Su, ZG. Preparation and characterization of dimeric bovine hemoglobin tetramers[J]. JOURNAL OF PROTEIN CHEMISTRY,2003,22(5):411-416.
APA Hu, T,Li, DX,&Su, ZG.(2003).Preparation and characterization of dimeric bovine hemoglobin tetramers.JOURNAL OF PROTEIN CHEMISTRY,22(5),411-416.
MLA Hu, T,et al."Preparation and characterization of dimeric bovine hemoglobin tetramers".JOURNAL OF PROTEIN CHEMISTRY 22.5(2003):411-416.
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