Knowledge Management System Of Institute of process engineering,CAS
| Preparation and structural regulation of macroporous agarose microspheres for highly efficient adsorption of giant biomolecules | |
| Zhao, Lan1; Huang, Lan1,2; Huang, Yongdong1; Zhu, Kai1; Che, Xiangjing1,3; Du, Yuxiang1,2; Gao, Jingwei1,2; Hao, Dongxia1; Zhang, Rongyue4; Wang, Qibao2; Ma, Guanghui1,5 | |
| 2022-06-01 | |
| Source Publication | COLLOID AND POLYMER SCIENCE
 |
| ISSN | 0303-402X |
| Volume | 300Issue:6Pages:691-705 |
| Abstract | Macroporous agarose microspheres for bioseparation of giant biomolecules were prepared by a surfactant micellar swelling method, and the effects of preparation conditions on both particle size and its distribution and pore structure were systematically studied. Under the optimal condition with a surfactant amount of 20% (v/v), Triton X-100 to sodium dodecyl sulfate ratio (SDS) of 5:1 (w/w), an oil-absorbing-swelling time of 2.5 h, and a stirring speed of 250 rpm, macroporous agarose microspheres were successfully prepared, characterized by inverse size exclusion chromatography. Distribution coefficient (K-d) of dextran probes for agarose microspheres after being cross-linked was almost the same as that for agarose microspheres before being cross-linked. Inverse size exclusion chromatography results showed that macroporous agarose microspheres had a pore size distribution of more than 40 nm or even close to 60 nm, while that of conventional microspheres was no more than 40 nm. Macroporous agarose microspheres functionalized with diethylaminoethyl groups had a higher adsorption capacity and faster adsorption rate for hepatitis B surface antigen (HBsAg) compared with both commercial DEAE-agarose chromatographic media and conventional DEAE-agarose microspheres which were both based on agarose microspheres prepared using a traditional method. Confocal laser scanning microscopy results showed that macroporous agarose microspheres had a good binding ability to HBsAg, and this antigen completely entered the inside of the microspheres. Compared with conventional agarose chromatographic media, the macroporous agarose media combined macromolecular proteins more quickly and fully, having wider applications in the field of purification of giant biomolecules. |
| Keyword | Macroporous agarose microspheres Micellar swelling method Pore-forming Chromatographic media Adsorption |
| DOI | 10.1007/s00396-022-04968-0 |
| Language | 英语 |
| WOS Keyword | SUPERPOROUS AGAROSE ; CHROMATOGRAPHY ; PURIFICATION ; MONOLITHS ; BEADS |
| Funding Project | National Key Research and Development Program of China[2021YFC2103402] ; National Key Research and Development Program of China[2021YFC2102802] ; National Key Research and Development Program of China[2018YFC0311101] ; Beijing Natural Science Foundation[L192017] ; National Natural Science Foundation of China[21821005] ; National Natural Science Foundation of China[22078334] ; National Natural Science Foundation of China[21902160] ; Youth Top Talent Project of Organization Department of Beijing Municipal Committee[ZZB2019001] |
| WOS Research Area | Chemistry ; Polymer Science |
| WOS Subject | Chemistry, Physical ; Polymer Science |
| Funding Organization | National Key Research and Development Program of China ; Beijing Natural Science Foundation ; National Natural Science Foundation of China ; Youth Top Talent Project of Organization Department of Beijing Municipal Committee |
| WOS ID | WOS:000792975700002 |
| Publisher | SPRINGER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/53293 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Huang, Yongdong; Ma, Guanghui |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.China Univ Min & Technol, Sch Chem & Environm Engn, Beijing 100083, Peoples R China 3.Yanshan Univ, Coll Environm & Chem Engn, Qinhuangdao 066000, Hebei, Peoples R China 4.Beijing Inst Petrochem Technol, Dept Appl Chem, Beijing 102617, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Zhao, Lan,Huang, Lan,Huang, Yongdong,et al. Preparation and structural regulation of macroporous agarose microspheres for highly efficient adsorption of giant biomolecules[J]. COLLOID AND POLYMER SCIENCE,2022,300(6):691-705. |
| APA | Zhao, Lan.,Huang, Lan.,Huang, Yongdong.,Zhu, Kai.,Che, Xiangjing.,...&Ma, Guanghui.(2022).Preparation and structural regulation of macroporous agarose microspheres for highly efficient adsorption of giant biomolecules.COLLOID AND POLYMER SCIENCE,300(6),691-705. |
| MLA | Zhao, Lan,et al."Preparation and structural regulation of macroporous agarose microspheres for highly efficient adsorption of giant biomolecules".COLLOID AND POLYMER SCIENCE 300.6(2022):691-705. |
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