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The preparation of an artificial red blood cell substitute by W/O/W double emulsion methods
Alternative TitleActa Biochim. Biophys. Sin.
Qiu, W; Meng, FT; Ma, GH; Su, ZG
2003-05-01
Source PublicationACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN0582-9879
Volume35Issue:5Pages:467-472
AbstractA method of preparing methoxypoly (ethylene glycol)-poly-DL-lactide (PELA) microcapsule containing bovine hemoglobin(BHb) by using W/O/W double emulsion-solvent diffusion process for use as artificial red cells was developed. The results suggested that preparation conditions such as solidification method, stirring rate, type of organic solvent had significant influence on the encapsulation efficiency and the activity of bovine hemoglobin. When ethyl acetate was used as the organic solvent and double emulsion-solvent diffusion process was used, there was no significant influence on the activity of hemoglobin if stirring rate was lower than 9000 r/min. High stirring rate, 12 000 r/min, decreased the P-50 and Hill coefficient of the hemoglobin encapsulated. Increasing the volume of solidification solution had an effect of improving the activity of hemoglobin to carry oxygen.; A method of preparing methoxypoly (ethylene glycol)-poly-DL-lactide (PELA) microcapsule containing bovine hemoglobin(BHb) by using W/O/W double emulsion-solvent diffusion process for use as artificial red cells was developed. The results suggested that preparation conditions such as solidification method, stirring rate, type of organic solvent had significant influence on the encapsulation efficiency and the activity of bovine hemoglobin. When ethyl acetate was used as the organic solvent and double emulsion-solvent diffusion process was used, there was no significant influence on the activity of hemoglobin if stirring rate was lower than 9000 r/min. High stirring rate, 12 000 r/min, decreased the P-50 and Hill coefficient of the hemoglobin encapsulated. Increasing the volume of solidification solution had an effect of improving the activity of hemoglobin to carry oxygen.
KeywordArtificial Red Cells Methoxypoly(Ethylene Glycol)-poly-dl-lactide Bovine Hemoglobin Microcapsule
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordMEMBRANE HEMOGLOBIN NANOCAPSULES ; POLY(LACTIC ACID) ; MICROSPHERES ; PROTEIN ; ENCAPSULATION ; PEG
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000183076800012
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5332
Collection研究所(批量导入)
AffiliationChinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Qiu, W,Meng, FT,Ma, GH,et al. The preparation of an artificial red blood cell substitute by W/O/W double emulsion methods[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(5):467-472.
APA Qiu, W,Meng, FT,Ma, GH,&Su, ZG.(2003).The preparation of an artificial red blood cell substitute by W/O/W double emulsion methods.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(5),467-472.
MLA Qiu, W,et al."The preparation of an artificial red blood cell substitute by W/O/W double emulsion methods".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.5(2003):467-472.
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