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Production of crocin using Crocus sativus callus by two-stage culture system
Alternative TitleBiotechnol. Lett.
Chen, SA; Wang, XD; Zhao, B; Yuan, XF; Wang, YC
2003-08-01
Source PublicationBIOTECHNOLOGY LETTERS
ISSN0141-5492
Volume25Issue:15Pages:1235-1238
AbstractSaffron callus was grown in a two-stage culture on B5 medium supplemented with casein hydrolysate ( 300 mg l(-1)) at 22 degreesC in dark with naphthalene acetic acid (2 mg l(-1)) and 6-benzyladenine (1 mg l(-1)) to give maximum biomass (16 g dry wt l(-1)), and with indole 3-acetic acid (2 mg l(-1)) and 6-benzyladenine (0.5 mg l(-1)) for crocin formation. The maximum crocin production (0.43 g l(-1)) was achieved by this two-stage culture method, which was three times that by a one-stage method.; Saffron callus was grown in a two-stage culture on B5 medium supplemented with casein hydrolysate ( 300 mg l(-1)) at 22 degreesC in dark with naphthalene acetic acid (2 mg l(-1)) and 6-benzyladenine (1 mg l(-1)) to give maximum biomass (16 g dry wt l(-1)), and with indole 3-acetic acid (2 mg l(-1)) and 6-benzyladenine (0.5 mg l(-1)) for crocin formation. The maximum crocin production (0.43 g l(-1)) was achieved by this two-stage culture method, which was three times that by a one-stage method.
KeywordCallus Crocin Crocus Sativas Optimization Two-stage System
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordTOBACCO TISSUE CULTURES ; REQUIREMENTS ; GROWTH
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000184578100005
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5340
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Chen, SA,Wang, XD,Zhao, B,et al. Production of crocin using Crocus sativus callus by two-stage culture system[J]. BIOTECHNOLOGY LETTERS,2003,25(15):1235-1238.
APA Chen, SA,Wang, XD,Zhao, B,Yuan, XF,&Wang, YC.(2003).Production of crocin using Crocus sativus callus by two-stage culture system.BIOTECHNOLOGY LETTERS,25(15),1235-1238.
MLA Chen, SA,et al."Production of crocin using Crocus sativus callus by two-stage culture system".BIOTECHNOLOGY LETTERS 25.15(2003):1235-1238.
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