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Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures
Alternative TitleBiotechnol. Lett.
Li, M; Poliakov, A; Danielson, UH; Su, ZG; Janson, JC
2003-10-01
Source PublicationBIOTECHNOLOGY LETTERS
ISSN0141-5492
Volume25Issue:20Pages:1729-1734
AbstractThe non-structural protein 3 (NS3) of Hepatitis C virus (HCV) was expressed as inactive aggregates in Escherichia coli. The protein was refolded by chromatographic techniques of which ion exchange chromatography was best for crude samples and gel filtration best for partially purified samples. Immobilized metal ion affinity chromatography showed intermediate performance. Gradient procedures enhanced the recovery of active NS3 protein.; The non-structural protein 3 (NS3) of Hepatitis C virus (HCV) was expressed as inactive aggregates in Escherichia coli. The protein was refolded by chromatographic techniques of which ion exchange chromatography was best for crude samples and gel filtration best for partially purified samples. Immobilized metal ion affinity chromatography showed intermediate performance. Gradient procedures enhanced the recovery of active NS3 protein.
KeywordGel Filtration Hcv Immobilized Metal Affinity Chromatography Ion-exchange Chromatography Ns3 Refolding
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordION-EXCHANGE CHROMATOGRAPHY ; PURIFICATION
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000185682000008
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5343
Collection研究所(批量导入)
Affiliation1.Uppsala Univ, Dept Surface Biotechnol, BMC, SE-75123 Uppsala, Sweden
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100864, Peoples R China
3.Uppsala Univ, Dept Biochem, BMC, SE-75123 Uppsala, Sweden
Recommended Citation
GB/T 7714
Li, M,Poliakov, A,Danielson, UH,et al. Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures[J]. BIOTECHNOLOGY LETTERS,2003,25(20):1729-1734.
APA Li, M,Poliakov, A,Danielson, UH,Su, ZG,&Janson, JC.(2003).Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures.BIOTECHNOLOGY LETTERS,25(20),1729-1734.
MLA Li, M,et al."Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures".BIOTECHNOLOGY LETTERS 25.20(2003):1729-1734.
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