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Separation and identification of different refolding components
Alternative TitleJ. Biotechnol.
Li, M; Su, ZG
2003-06-26
Source PublicationJOURNAL OF BIOTECHNOLOGY
ISSN0168-1656
Volume103Issue:2Pages:119-127
AbstractA dual-gradient ion-exchange chromatography (IEC) refolding process has been successfully applied to renature lysozyme at high concentration. The different refolding components were separated by size-exclusion chromatography with low concentrations of urea and NaCl added into elution buffer. The hydrodynamic characteristics during separation and the hydrophobic properties of these components were also tested. (C) 2003 Elsevier Science B.V. All rights reserved.; A dual-gradient ion-exchange chromatography (IEC) refolding process has been successfully applied to renature lysozyme at high concentration. The different refolding components were separated by size-exclusion chromatography with low concentrations of urea and NaCl added into elution buffer. The hydrodynamic characteristics during separation and the hydrophobic properties of these components were also tested. (C) 2003 Elsevier Science B.V. All rights reserved.
KeywordSize-exclusion Chromatography Ion-exchange Chromatography Hydrophobic Interaction Chromatography Refolding Aggregation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/S0168-1656(03)00108-1
URL查看原文
Indexed BySCI
Language英语
WOS KeywordSIZE-EXCLUSION CHROMATOGRAPHY ; HUMAN INTERFERON-GAMMA ; DENATURED/REDUCED LYSOZYME ; DISEASE ; RENATURATION ; AGGREGATION ; ALZHEIMERS
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000183825000004
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5344
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Li, M,Su, ZG. Separation and identification of different refolding components[J]. JOURNAL OF BIOTECHNOLOGY,2003,103(2):119-127.
APA Li, M,&Su, ZG.(2003).Separation and identification of different refolding components.JOURNAL OF BIOTECHNOLOGY,103(2),119-127.
MLA Li, M,et al."Separation and identification of different refolding components".JOURNAL OF BIOTECHNOLOGY 103.2(2003):119-127.
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