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A solid phase adsorption method for preparation of bovine serum albumin-bovine hemoglobin conjugate
Alternative TitleJ. Biotechnol.
Hu, T; Su, ZG
2003-02-13
Source PublicationJOURNAL OF BIOTECHNOLOGY
ISSN0168-1656
Volume100Issue:3Pages:267-275
AbstractA solid phase adsorption method was proposed to prepare well-defined bovine serum albumin-bovine hemoglobin (Hb) conjugate. After adsorption by the solid phase, Q Sepharose Fast Flow media, bovine serum albumin (BSA) molecules were allowed to react with glutaraldehyde. The spacing out of BSA molecules on the solid phase was assumed to limit polymerization of BSA molecules, except some molecules bound closely on the solid phase resulting in minor dimer formation. Following the elution procedure, the activated monomeric BSA was separated from the dimers by gel filtration chromatography on Superdex 200 and then reacted with bovine Hb at 4 degreesC and pH 9.5. The 1:1 (BSA:Hb) conjugate was obtained with the yield of 64%. The P-50 values of the conjugates, prepared under anaerobic and aerobic conditions, were 19.1 and 14.2 mmHg, respectively. The dependence of the P-50 on chloride ions for the conjugate was slightly diminished, presumably due to covalent attachment of BSA to bovine Hb. (C) 2002 Elsevier Science B.V. All rights reserved.; A solid phase adsorption method was proposed to prepare well-defined bovine serum albumin-bovine hemoglobin (Hb) conjugate. After adsorption by the solid phase, Q Sepharose Fast Flow media, bovine serum albumin (BSA) molecules were allowed to react with glutaraldehyde. The spacing out of BSA molecules on the solid phase was assumed to limit polymerization of BSA molecules, except some molecules bound closely on the solid phase resulting in minor dimer formation. Following the elution procedure, the activated monomeric BSA was separated from the dimers by gel filtration chromatography on Superdex 200 and then reacted with bovine Hb at 4 degreesC and pH 9.5. The 1:1 (BSA:Hb) conjugate was obtained with the yield of 64%. The P-50 values of the conjugates, prepared under anaerobic and aerobic conditions, were 19.1 and 14.2 mmHg, respectively. The dependence of the P-50 on chloride ions for the conjugate was slightly diminished, presumably due to covalent attachment of BSA to bovine Hb. (C) 2002 Elsevier Science B.V. All rights reserved.
KeywordBlood Substitute Bovine Hemoglobin Bovine Serum Albumin Glutaraldehyde Solid Phase Adsorption
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
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Indexed BySCI
Language英语
WOS KeywordOXYGEN
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000180578500010
Citation statistics
Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5350
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Hu, T,Su, ZG. A solid phase adsorption method for preparation of bovine serum albumin-bovine hemoglobin conjugate[J]. JOURNAL OF BIOTECHNOLOGY,2003,100(3):267-275.
APA Hu, T,&Su, ZG.(2003).A solid phase adsorption method for preparation of bovine serum albumin-bovine hemoglobin conjugate.JOURNAL OF BIOTECHNOLOGY,100(3),267-275.
MLA Hu, T,et al."A solid phase adsorption method for preparation of bovine serum albumin-bovine hemoglobin conjugate".JOURNAL OF BIOTECHNOLOGY 100.3(2003):267-275.
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