CAS OpenIR  > 研究所(批量导入)
Stability of tumor necrosis factor-alpha during ion exchange chromatography
Alternative TitleBiotechnol. Lett.
Li, M; Wang, YS; Li, WJ; Su, ZG
2003-06-01
Source PublicationBIOTECHNOLOGY LETTERS
ISSN0141-5492
Volume25Issue:11Pages:905-908
AbstractDenaturation of target protein by various separation and purification steps contributes significant part to the total product loss in bioseparation. The conformational change and accompanying loss of activity of tumor necrosis factor-alpha during ion exchange chromatography was reversible and was decreased by adding polyethylene glycol 200 at 2 to 5% (v/v) to the eluting solution.; Denaturation of target protein by various separation and purification steps contributes significant part to the total product loss in bioseparation. The conformational change and accompanying loss of activity of tumor necrosis factor-alpha during ion exchange chromatography was reversible and was decreased by adding polyethylene glycol 200 at 2 to 5% (v/v) to the eluting solution.
KeywordConformational Change Ion Exchange Chromatography Polyethylene Glycol
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPURIFICATION
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000183320800015
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5355
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100864, Peoples R China
Recommended Citation
GB/T 7714
Li, M,Wang, YS,Li, WJ,et al. Stability of tumor necrosis factor-alpha during ion exchange chromatography[J]. BIOTECHNOLOGY LETTERS,2003,25(11):905-908.
APA Li, M,Wang, YS,Li, WJ,&Su, ZG.(2003).Stability of tumor necrosis factor-alpha during ion exchange chromatography.BIOTECHNOLOGY LETTERS,25(11),905-908.
MLA Li, M,et al."Stability of tumor necrosis factor-alpha during ion exchange chromatography".BIOTECHNOLOGY LETTERS 25.11(2003):905-908.
Files in This Item:
File Name/Size DocType Version Access License
Stability of tumor n(69KB) 限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, M]'s Articles
[Wang, YS]'s Articles
[Li, WJ]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, M]'s Articles
[Wang, YS]'s Articles
[Li, WJ]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, M]'s Articles
[Wang, YS]'s Articles
[Li, WJ]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.