CAS OpenIR  > 研究所(批量导入)
W/O/W double emulsion technique using ethyl acetate as organic solvent: effects of its diffusion rate on the characteristics of microparticles
Alternative TitleJ. Control. Release
Meng, FT; Ma, GH; Qiu, W; Su, ZG
2003-09-04
Source PublicationJOURNAL OF CONTROLLED RELEASE
ISSN0168-3659
Volume91Issue:3Pages:407-416
AbstractMonomethoxypoly (ethylene glycol)-b-poly(DL-lactide) copolymer (PELA) microparticles loading lysozyme were prepared through a modified W/O/W double emulsion-solvent diffusion method using ethyl acetate (EA) as organic solvent. The modified process was divided into five steps: (1) primary emulsification (W-1/O), (2) re-emulsification (W-1/O/W-2), (3) pre-solidification, (4) solidification and (5) purification. The pre-solidification step was carried out in the modified process to control the diffusion rate of EA from oil phase into outer aqueous phase, in order to prevent the wall polymer from precipitation, which usually occurred when the diffusion rate was too fast. The adequately rapid solidification of microparticle caused by controlled fast diffusion of EA and the use of amphiphilic copolymer PELA as wall material, facilitated a high protein entrapment (always above 94%) and full preservation of bioactivity of entrapped lysozyme. It was found that the volume of the outer aqueous phase in the re-emulsification step and the shear stress in the pre-solidification step had a significant effect on the diffusion rate of EA from the droplets into outer aqueous solution, and thereby on the characteristics of the resultant microparticles. With the volume or the shear stress increasing, the removal rate of EA increased, resulting in rapid solidification of the microparticles. This result led to a lower burst effect and a slower lysozyme release from the microparticles. This study suggests that the modified W/O/W double emulsion-solvent diffusion method with EA as organic solvent is a prospective technique to prepare biodegradable microparticles containing water-soluble sensitive agents. (C) 2003 Elsevier B.V. All rights reserved.; Monomethoxypoly (ethylene glycol)-b-poly(DL-lactide) copolymer (PELA) microparticles loading lysozyme were prepared through a modified W/O/W double emulsion-solvent diffusion method using ethyl acetate (EA) as organic solvent. The modified process was divided into five steps: (1) primary emulsification (W-1/O), (2) re-emulsification (W-1/O/W-2), (3) pre-solidification, (4) solidification and (5) purification. The pre-solidification step was carried out in the modified process to control the diffusion rate of EA from oil phase into outer aqueous phase, in order to prevent the wall polymer from precipitation, which usually occurred when the diffusion rate was too fast. The adequately rapid solidification of microparticle caused by controlled fast diffusion of EA and the use of amphiphilic copolymer PELA as wall material, facilitated a high protein entrapment (always above 94%) and full preservation of bioactivity of entrapped lysozyme. It was found that the volume of the outer aqueous phase in the re-emulsification step and the shear stress in the pre-solidification step had a significant effect on the diffusion rate of EA from the droplets into outer aqueous solution, and thereby on the characteristics of the resultant microparticles. With the volume or the shear stress increasing, the removal rate of EA increased, resulting in rapid solidification of the microparticles. This result led to a lower burst effect and a slower lysozyme release from the microparticles. This study suggests that the modified W/O/W double emulsion-solvent diffusion method with EA as organic solvent is a prospective technique to prepare biodegradable microparticles containing water-soluble sensitive agents. (C) 2003 Elsevier B.V. All rights reserved.
KeywordProtein Delivery Stability Solvent Diffusion Method Ethyl Acetate Microparticle
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine
DOI10.1016/S0168-3659(03)0273-6
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPOLY(D,L-LACTIC-CO-GLYCOLIC ACID) MICROPARTICLES ; HUMAN GROWTH-HORMONE ; BIODEGRADABLE MICROSPHERES ; PROTEIN INSTABILITY ; BREAKUP MECHANISMS ; PLGA MICROSPHERES ; MICROENCAPSULATION ; EMULSIFICATION ; NANOPARTICLES ; FORMULATIONS
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Multidisciplinary ; Pharmacology & Pharmacy
WOS IDWOS:000185376100012
Citation statistics
Cited Times:118[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5370
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Meng, FT,Ma, GH,Qiu, W,et al. W/O/W double emulsion technique using ethyl acetate as organic solvent: effects of its diffusion rate on the characteristics of microparticles[J]. JOURNAL OF CONTROLLED RELEASE,2003,91(3):407-416.
APA Meng, FT,Ma, GH,Qiu, W,&Su, ZG.(2003).W/O/W double emulsion technique using ethyl acetate as organic solvent: effects of its diffusion rate on the characteristics of microparticles.JOURNAL OF CONTROLLED RELEASE,91(3),407-416.
MLA Meng, FT,et al."W/O/W double emulsion technique using ethyl acetate as organic solvent: effects of its diffusion rate on the characteristics of microparticles".JOURNAL OF CONTROLLED RELEASE 91.3(2003):407-416.
Files in This Item:
File Name/Size DocType Version Access License
W_O_W double emulsio(446KB) 限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Meng, FT]'s Articles
[Ma, GH]'s Articles
[Qiu, W]'s Articles
Baidu academic
Similar articles in Baidu academic
[Meng, FT]'s Articles
[Ma, GH]'s Articles
[Qiu, W]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Meng, FT]'s Articles
[Ma, GH]'s Articles
[Qiu, W]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.