CAS OpenIR  > 生化工程国家重点实验室
Salt Induced Irreversible Protein Adsorption with Extremely High Loadings on Electrospun Nanofibers
Liu, Chun-Xia1,2; Zhang, Song-Ping1; Su, Zhi-Guo1; Wang, Ping3,4; Su, ZG
2011-01-18
Source PublicationLANGMUIR
ISSN0743-7463
Volume27Issue:2Pages:760-765
AbstractLiCl is a kosmotrope that generally promotes protein salvation in aqueous solutions. Herein we report that LiCl embedded in electrospun polymeric nanofibers interestingly induced an abnormal protein adsorption and substantially augmented the adsorption capacity of the fibers. As a result, equilibrium protein loadings reached over 64% (w/w) of the dry mass of fibers, 9-fold higher than that observed in the absence of the salt. The adsorption appeared to be irreversible such that little protein loss was observed even after washing the fibers vigorously with fresh buffer solutions. We further examined the application of such intensified protein adsorption for enzyme immobilization. Proteins including bovine serum albumin (BSA) and prolamine were first adsorbed, followed by covalent attachment of an outer layer of an enzyme, alpha-chymotrypsin. Such a multilayer-structured nanofibrous enzyme exhibited extremely high stability with no obvious activity loss even after being incubated for 8 months at 4 degrees C in aqueous buffer solution. The LiCl induced irreversible protein adsorption, which has been largely ignored in previous studies with electrospun materials, rendering an interesting scenario of interfacial protein-material interactions. It also reveals a new mechanism in controlling and fabricating molecular interactions at interfaces for development of a broad range of biomaterials.
KeywordPolymer Nanofibres Metal-ions Behavior Immobilization Biocatalysts Desorption Stability Membranes Biomass
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
DOI10.1021/la103392e
Indexed BySCI
Language英语
WOS KeywordPOLYMER NANOFIBRES ; METAL-IONS ; BEHAVIOR ; IMMOBILIZATION ; BIOCATALYSTS ; DESORPTION ; STABILITY ; MEMBRANES ; BIOMASS
WOS Research AreaChemistry ; Materials Science
WOS SubjectChemistry, Multidisciplinary ; Chemistry, Physical ; Materials Science, Multidisciplinary
WOS IDWOS:000285990500038
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5376
Collection生化工程国家重点实验室
Corresponding AuthorSu, ZG
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA
4.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA
Recommended Citation
GB/T 7714
Liu, Chun-Xia,Zhang, Song-Ping,Su, Zhi-Guo,et al. Salt Induced Irreversible Protein Adsorption with Extremely High Loadings on Electrospun Nanofibers[J]. LANGMUIR,2011,27(2):760-765.
APA Liu, Chun-Xia,Zhang, Song-Ping,Su, Zhi-Guo,Wang, Ping,&Su, ZG.(2011).Salt Induced Irreversible Protein Adsorption with Extremely High Loadings on Electrospun Nanofibers.LANGMUIR,27(2),760-765.
MLA Liu, Chun-Xia,et al."Salt Induced Irreversible Protein Adsorption with Extremely High Loadings on Electrospun Nanofibers".LANGMUIR 27.2(2011):760-765.
Files in This Item:
File Name/Size DocType Version Access License
20131108-1041-复件 la1(2754KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, Chun-Xia]'s Articles
[Zhang, Song-Ping]'s Articles
[Su, Zhi-Guo]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, Chun-Xia]'s Articles
[Zhang, Song-Ping]'s Articles
[Su, Zhi-Guo]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, Chun-Xia]'s Articles
[Zhang, Song-Ping]'s Articles
[Su, Zhi-Guo]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.