Gelatin/sodium alginate hydrogel-coated decellularized porcine coronary to construct tissue blood vessels
Du, Jing1; Hu, Xueyan1; Su, Ya1; Wei, Tuo1; Jiao, Zeren2; Liu, Tianqing1; Wang, Hong3; Nie, Yi4,5; Li, Xiangqin1; Song, Kedong1
AbstractCardiovascular diseases and vascular trauma can be commonly found in the population. Scholars worldwide hope to develop small-diameter vascular grafts that can replace autologous vessels for clinical use. Decellularized blood vessels can retain the original morphology, structure, and physical properties of blood vessels, which is conducive to cell growth, proliferation, and differentiation. In this study, porcine coronary arteries (PCAs) were decellularized to prepare decellularized porcine coronary artery (DPCA), and bilayer hybrid scaffolds were prepared by coating gelatin and sodium alginate mixed hydrogel of seven different proportions and combined with mouse fibroblasts (L929 cells) to study the construction of tissue engineering vessels in vitro. The obtained bilayer hybrid scaffolds were 3-7 cm in length, 5 mm in external diameter, and 1 mm in average wall thickness. All seven bilayer hybrid scaffolds showed good biocompatibility after cell inoculation. Compared with 2D culture, cells on 3D scaffolds grew relatively slowly in the first 4 days, and the number of cells proliferated rapidly at 7 days. In the same culture days, different concentrations of hydrogel also had an impact on cell proliferation. With the increase of hydrogel content, cells on the 3D scaffold formed cell colonies faster. The results showed that the scaffold had good biocompatibility and could meet the needs of artificial blood vessel construction.
KeywordDecellularized porcine coronary artery Gelatin sodium alginate Bilayer scaffolds construct Fibroblasts Tissue engineered blood vessels
Funding ProjectNational Natural Science Foundation of China[31670978/31971275] ; Fok Ying Tung Education Foun-dation[132027] ; State Key Laboratory of Fine Chemicals[KF1111] ; Natural Science Foundation of Liaoning[20180510028] ; Fundamental Research Funds for the Central Universities[DUT20ZD206/DUT20YG118/DUT21YG113]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
Funding OrganizationNational Natural Science Foundation of China ; Fok Ying Tung Education Foun-dation ; State Key Laboratory of Fine Chemicals ; Natural Science Foundation of Liaoning ; Fundamental Research Funds for the Central Universities
WOS IDWOS:000805954000001
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Document Type期刊论文
Corresponding AuthorWang, Hong; Nie, Yi; Li, Xiangqin; Song, Kedong
Affiliation1.Dalian Univ Technol, Dalian R&D Ctr Stem Cell& Tissue Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China
2.Texas A&M Univ, Artie McFerrin Dept Chem Engn, College Stn, TX 77843 USA
3.Dalian Med Univ, Dept Orthopead, Dalian Municipal Cent Hosp, Dalian 116033, Peoples R China
4.Zhengzhou Inst Emerging Ind Technol, Zhengzhou 450000, Peoples R China
5.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
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GB/T 7714
Du, Jing,Hu, Xueyan,Su, Ya,et al. Gelatin/sodium alginate hydrogel-coated decellularized porcine coronary to construct tissue blood vessels[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2022,209:2070-2083.
APA Du, Jing.,Hu, Xueyan.,Su, Ya.,Wei, Tuo.,Jiao, Zeren.,...&Song, Kedong.(2022).Gelatin/sodium alginate hydrogel-coated decellularized porcine coronary to construct tissue blood vessels.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,209,2070-2083.
MLA Du, Jing,et al."Gelatin/sodium alginate hydrogel-coated decellularized porcine coronary to construct tissue blood vessels".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 209(2022):2070-2083.
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