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Simultaneous production of 1,3-dihydroxyacetone and xylitol from glycerol and xylose using a nanoparticle-supported multi-enzyme system with in situ cofactor regeneration
Zhang, Ying1,2; Gao, Fei1,2; Zhang, Song-Ping1; Su, Zhi-Guo1; Ma, Guang-Hui1; Wang, Ping3,4; Ma, GH
2011
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume102Issue:2Pages:1837-1843
AbstractCofactor-dependent biotransformations often require consumption of a secondary substrate for cofactor regeneration. Alternatively, two synthetic reactions may be coupled together through cofactor regeneration cycles. Simultaneous production of value-added products from glycerol and xylose was realized in this work through an enzymatic NAD(H) regeneration cycle involving two enzymes. Glycerol dehydrogenase (GDH) catalyzed the production of 1,3-dihydroxyacetone (DHA) from glycerol, while xylose reductase (XR) enabled the reduction of xylose to xylitol using the protons released from glycerol. Both enzymes were immobilized with P(MMA-EDMA-MAA) nanoparticles. Interestingly, the immobilized multi-enzyme system showed much improved productivity and stability as compared to native enzymes, such that the total turnover number (TTN) reached 82 for cofactor regeneration while the yield reached 160 g/g-immobilized GDH for DHA production. (C) 2010 Elsevier Ltd. All rights reserved.
KeywordEnzymatic Biocatalysis Cofactor Regeneration Glycerol Dehydrogenase Xylose Reductase Biosynthesis
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.biortech.2010.09.069
Indexed BySCI
Language英语
WOS KeywordBIOCATALYTIC ACTIVITY ; CARBON NANOTUBES ; MEMBRANE REACTOR ; SINGLE-ENZYME ; DEHYDROGENASE ; NANOFIBERS ; STABILITY ; ACID
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000286782700196
Citation statistics
Cited Times:52[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5383
Collection生化工程国家重点实验室
Corresponding AuthorMa, GH
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA
4.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA
Recommended Citation
GB/T 7714
Zhang, Ying,Gao, Fei,Zhang, Song-Ping,et al. Simultaneous production of 1,3-dihydroxyacetone and xylitol from glycerol and xylose using a nanoparticle-supported multi-enzyme system with in situ cofactor regeneration[J]. BIORESOURCE TECHNOLOGY,2011,102(2):1837-1843.
APA Zhang, Ying.,Gao, Fei.,Zhang, Song-Ping.,Su, Zhi-Guo.,Ma, Guang-Hui.,...&Ma, GH.(2011).Simultaneous production of 1,3-dihydroxyacetone and xylitol from glycerol and xylose using a nanoparticle-supported multi-enzyme system with in situ cofactor regeneration.BIORESOURCE TECHNOLOGY,102(2),1837-1843.
MLA Zhang, Ying,et al."Simultaneous production of 1,3-dihydroxyacetone and xylitol from glycerol and xylose using a nanoparticle-supported multi-enzyme system with in situ cofactor regeneration".BIORESOURCE TECHNOLOGY 102.2(2011):1837-1843.
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