| Preparation and evaluation of alginate-chitosan microspheres for oral delivery of insulin | |
| Zhang, Yueling1,2; Wei, Wei1,2; Lv, Piping1,2; Wang, Lianyan1; Ma, Guanghui1; Ma, GH | |
| 2011 | |
| Source Publication | EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
 |
| ISSN | 0939-6411 |
| Volume | 77Issue:1Pages:11-19 |
| Abstract | The alginate-chitosan microspheres with narrow size distribution were prepared by membrane emulsification technique in combination with ion (Ca(2+)) and polymer (chitosan) solidification. The preparation procedure was observed, and the physical properties (particle size distribution, surface morphology, chitosan distribution, zeta potential) of the microspheres were characterized. Subsequently, the microspheres were employed to load model peptide of insulin. The effect of loading ways on the loading efficiency and immunological activity of insulin were investigated. It was shown that the higher loading efficiency (56.7%) and remarkable activity maintenance (99.4%) were obtained when the insulin was loaded during the chitosan solidification process (Method B). Afterward, the release profile in vitro for the optimal insulin-loaded microspheres was investigated. Under the pH conditions of gastrointestinal environment, only 32% of insulin released during the simulated transit time of drug (2 h in the stomach and 4 h in the intestinal). While under the pH condition of blood environment, insulin release was stable and sustained for a long time (14 days). Furthermore, the chemical stability of insulin released from the microspheres was well preserved after they were treated with the simulated gastric fluid containing pepsin for 2 h. Finally, the blood glucose level of diabetic rats could be effectively reduced and stably kept for a long time (similar to 60 h) after oral administration of the insulin-loaded alginate-chitosan microspheres. Therefore, the alginate-chitosan microspheres were found to be promising vectors showing a good efficiency in oral administration of protein or peptide drugs. (C) 2010 Elsevier B.V. All rights reserved. |
| Keyword | Narrow Size Distribution Alginate-chitosan Microspheres Protein Or Peptide Oral Administration Insulin Sustained Release |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1016/j.ejpb.2010.09.016 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | DRUG-DELIVERY ; PROTEIN DRUGS ; CONTROLLED-RELEASE ; INTERNAL GELATION ; SODIUM ALGINATE ; GEL BEADS ; CARRIER ; PEPTIDE ; SYSTEM ; RAT |
| WOS Research Area | Pharmacology & Pharmacy |
| WOS Subject | Pharmacology & Pharmacy |
| WOS ID | WOS:000286687500003 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/5387 |
| Collection | 生化工程国家重点实验室 |
| Corresponding Author | Ma, GH |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Yueling,Wei, Wei,Lv, Piping,et al. Preparation and evaluation of alginate-chitosan microspheres for oral delivery of insulin[J]. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,2011,77(1):11-19. |
| APA | Zhang, Yueling,Wei, Wei,Lv, Piping,Wang, Lianyan,Ma, Guanghui,&Ma, GH.(2011).Preparation and evaluation of alginate-chitosan microspheres for oral delivery of insulin.EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,77(1),11-19. |
| MLA | Zhang, Yueling,et al."Preparation and evaluation of alginate-chitosan microspheres for oral delivery of insulin".EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS 77.1(2011):11-19. |
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| File Name/Size | DocType | Version | Access | License | ||
| 20131108-1055-复件 1-s（1091KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | Application Full Text | |
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