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Bioinspired preparation of polydopamine microcapsule for multienzyme system construction
Zhang, Lei1,2; Shi, Jiafu1,2; Jiang, Zhongyi1,2; Jiang, Yanjun3; Qiao, Shizhang4; Li, Jian1; Wang, Rui1; Meng, Ruijie1; Zhu, Yuanyuan1; Zheng, Yang1; Zhang, L
2011
Source PublicationGREEN CHEMISTRY
ISSN1463-9262
Volume13Issue:2Pages:300-306
AbstractInspired by the structural organization of mitochondria and the bioadhesive principle, a simple and versatile approach to construct a multienzyme system is developed. More specifically, the multienzyme system is composed of a polydopamine (PDA) microcapsule scaffold and three spatially separated enzymes. The PDA microcapsules are prepared through the rapid, spontaneous self-polymerization of dopamine on the surface of CaCO(3) microparticle template, followed by dissolution of the template using EDTA. The wall thickness of the microcapsules can be tuned by the dopamine concentration in an aqueous solution. The three enzymes are respectively immobilized through physical encapsulation in the lumen, in situ entrapment within the wall and chemical attachment on the out surface under extremely mild conditions. As an example, a multienzyme system, containing alpha-amylase, beta-amylase and glucosidase, was constructed to convert starch into isomaltooligosaccharide, and the multienzyme system displays higher catalytic activity and enhanced operational stability. The method developed in this study will establish a powerful platform for the facile construction of multienzyme cascade systems.
KeywordSurface-chemistry Polymer Capsules Polyelectrolyte Enzymes Microparticles Encapsulation Templates Particles Dopamine Coatings
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1039/c0gc00432d
Indexed BySCI
Language英语
WOS KeywordSURFACE-CHEMISTRY ; POLYMER CAPSULES ; POLYELECTROLYTE ; ENZYMES ; MICROPARTICLES ; ENCAPSULATION ; TEMPLATES ; PARTICLES ; DOPAMINE ; COATINGS
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000287092500010
Citation statistics
Cited Times:127[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5388
Collection生化工程国家重点实验室
Corresponding AuthorZhang, L
Affiliation1.Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Key Lab Green Technol, Tianjin 300072, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Hebei Univ Technol, Sch Chem Engn, Dept Bioengn, Tianjin 300130, Peoples R China
4.Univ Queensland, Australian Inst Bioengn & Nanotechnol, ARC Ctr Excellence Funct Nanomat, Brisbane, Qld 4072, Australia
Recommended Citation
GB/T 7714
Zhang, Lei,Shi, Jiafu,Jiang, Zhongyi,et al. Bioinspired preparation of polydopamine microcapsule for multienzyme system construction[J]. GREEN CHEMISTRY,2011,13(2):300-306.
APA Zhang, Lei.,Shi, Jiafu.,Jiang, Zhongyi.,Jiang, Yanjun.,Qiao, Shizhang.,...&Zhang, L.(2011).Bioinspired preparation of polydopamine microcapsule for multienzyme system construction.GREEN CHEMISTRY,13(2),300-306.
MLA Zhang, Lei,et al."Bioinspired preparation of polydopamine microcapsule for multienzyme system construction".GREEN CHEMISTRY 13.2(2011):300-306.
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