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Melatonin improves the survival of cryopreserved callus of Rhodiola crenulata
Zhao, Yan1,2; Qi, Li-Wang3; Wang, Wei-Ming4; Saxena, Praveen K.5; Liu, Chun-Zhao1,2; Liu, CZ
2011
Source PublicationJOURNAL OF PINEAL RESEARCH
ISSN0742-3098
Volume50Issue:1Pages:83-88
AbstractAn important aspect of the function of melatonin seems to be the mediation of stress caused by environmental and chemical factors. In the cryopreservation process, environmental changes including osmotic injury, desiccation, and low temperature can impose a series of stresses on plants. In this study, we evaluated the role of melatonin in stress protection during the process of cryopreservation using callus of an endangered plant species Rhodiola crenulata. The survival rate of the cryopreserved callus significantly increased when the callus was pretreated for 5 days with 0.1 mu m melatonin prior to freezing in liquid nitrogen. Analysis of antioxidative activity following the pretreatment of callus with 0.1 mu m melatonin showed a significant reduction in malondialdehyde production during various steps of cryopreservation. Enhanced peroxidase and catalase activity was observed in the callus after pretreatment with 0.1 mu m melatonin compared to the control. These observations provide new evidence of the antioxidant/anti-stress function of melatonin, and it is the first report of its potential application in the preservation of elite endangered germplasm through the process of cryopreservation.
KeywordAntioxidative Enzymes Cryopreservation Malondialdehyde Melatonin Rhodiola Crenulata
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1111/j.1600-079X.2010.00817.x
Indexed BySCI
Language英语
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; SHOOT-TIPS ; SUPEROXIDE-DISMUTASE ; ANTIOXIDANT ENZYMES ; PEROXIDASE-ACTIVITY ; OXIDATIVE STRESS ; EUGLENA-GRACILIS ; VITRIFICATION ; PLANTS ; CELLS
WOS Research AreaEndocrinology & Metabolism ; Neurosciences & Neurology ; Physiology
WOS SubjectEndocrinology & Metabolism ; Neurosciences ; Physiology
WOS IDWOS:000285154400011
Citation statistics
Cited Times:75[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5400
Collection生化工程国家重点实验室
Corresponding AuthorLiu, CZ
Affiliation1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China
3.Chinese Acad Forestry, Res Inst Forestry, Beijing, Peoples R China
4.Phyton Biotech Inc, E Windsor, NJ USA
5.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada
Recommended Citation
GB/T 7714
Zhao, Yan,Qi, Li-Wang,Wang, Wei-Ming,et al. Melatonin improves the survival of cryopreserved callus of Rhodiola crenulata[J]. JOURNAL OF PINEAL RESEARCH,2011,50(1):83-88.
APA Zhao, Yan,Qi, Li-Wang,Wang, Wei-Ming,Saxena, Praveen K.,Liu, Chun-Zhao,&Liu, CZ.(2011).Melatonin improves the survival of cryopreserved callus of Rhodiola crenulata.JOURNAL OF PINEAL RESEARCH,50(1),83-88.
MLA Zhao, Yan,et al."Melatonin improves the survival of cryopreserved callus of Rhodiola crenulata".JOURNAL OF PINEAL RESEARCH 50.1(2011):83-88.
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