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Particle size affects the cellular response in macrophages
Yue, Hua1,2; Wei, Wei1,2; Yue, Zhanguo1,2; Lv, Piping1,2; Wang, Lianyan1; Ma, Guanghui1; Su, Zhiguo1; Ma, GH
2010-12-23
Source PublicationEUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
ISSN0928-0987
Volume41Issue:5Pages:650-657
AbstractA deeper understanding of how the physical properties of particles regulate specific biological responses is becoming a crucial requirement for their successful biomedical application. To provide insights on their design and application, J774A.1 cells are exposed to particles with different diameters (430 nm, 1.9 mu m and 4.8 mu m), and the size effects on a series of cellular responses in macrophages are evaluated. Cellular uptake study demonstrates that nanosized particles accumulate in the cells at a faster rate, and with a higher surface area. Once the data are converted into the expression of particle volume, the maximum value is found with 1.9 mu m particles instead of nanoparticles. Moreover, the uptake intermediates are also trapped, and the steps of particle internalization include filopodia sensing, skeleton rearrangement, and morphology change. Subsequent cellular trafficking reveals that only nanosized particles transport via lysosomal pathway, which is consistent with their uptake mechanisms. Furthermore, nanosized particles prefer to promote the secretion of Th1-specific molecule signals (e.g. IFN, IL-12) rather than immune suppressors. All these results, along with a couple of surprises, are discussed in the view of clinical practice. They are expected, in principle, to establish the basis of new design concepts for particle-based biomedical applications. (C) 2010 Elsevier B.V. All rights reserved.
KeywordBiomaterials Cellular Response Macrophage Particle Design Size
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.ejps.2010.09.006
Indexed BySCI
Language英语
WOS KeywordDRUG-DELIVERY ; GOLD NANOPARTICLES ; SURFACE-PROPERTIES ; VACCINE ADJUVANTS ; DENDRITIC CELLS ; PATHWAYS ; DESIGN ; INTERNALIZATION ; MICROSPHERES ; EXPRESSION
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000285451300011
Citation statistics
Cited Times:102[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5406
Collection生化工程国家重点实验室
Corresponding AuthorMa, GH
Affiliation1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yue, Hua,Wei, Wei,Yue, Zhanguo,et al. Particle size affects the cellular response in macrophages[J]. EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES,2010,41(5):650-657.
APA Yue, Hua.,Wei, Wei.,Yue, Zhanguo.,Lv, Piping.,Wang, Lianyan.,...&Ma, GH.(2010).Particle size affects the cellular response in macrophages.EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES,41(5),650-657.
MLA Yue, Hua,et al."Particle size affects the cellular response in macrophages".EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES 41.5(2010):650-657.
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