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Application of a pH Feedback-Controlled Substrate Feeding Method in Lactic Acid Production
Zhang, Yong1,2; Cong, Wei1,2; Shi, ShaoYuan1,2; Cong, W
2010-12-01
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN0273-2289
Volume162Issue:8Pages:2149-2156
AbstractSubstrate concentration in lactic acid fermentation broth could not be controlled well by traditional feeding methods, including constant, intermittent, and exponential feeding methods, in fed-batch experiments. A simple feedback feeding method based on pH was proposed to control pH and substrate concentration synchronously to enhance lactic acid production in fed-batch culture. As the linear relationship between the consumption amounts of alkali and that of substrate was concluded during lactic acid fermentation, the alkali and substrate in the feeding broth were mixed together proportionally. Thus, the concentration of substrate could be controlled through the adjustment of pH automatically. In the fed-batch lactic acid fermentation with Lactobacillus lactis-11 by this method, the residual glucose concentration in fermentation broth was controlled between 4.1 and 4.9 g L-1, and the highest concentration of lactic acid, maximum cell dry weight, volumetric productivity of lactic acid, and yield were 96.3 g L-1, 4.7 g L-1, 1.9 g L-1 h(-1) and 0.99 g lactic acid per gram of glucose, respectively, compared to 82.7 g L-1, 3.31 g L-1 1.7 g L-1 h(-1) and 0.92 g lactic acid per gram of glucose in batch culture. This feeding method was simple and easily operated and could be feasible for industrial lactic acid production in the future.
KeywordLactic Acid Fed-batch Lactobacillus Lactis-11 Ammonium Hydroxide Ph Feedback
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s12010-010-8989-x
Indexed BySCI
Language英语
WOS KeywordLACTOBACILLUS-AMYLOPHILUS GV6 ; FED-BATCH CULTURE ; RHIZOPUS-ORYZAE ; SIMULTANEOUS SACCHARIFICATION ; DIRECT FERMENTATION ; AMMONIUM LACTATE ; SUBSP-LACTIS ; STARCH ; BROTH
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000284295000003
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5411
Collection生化工程国家重点实验室
Corresponding AuthorCong, W
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Yong,Cong, Wei,Shi, ShaoYuan,et al. Application of a pH Feedback-Controlled Substrate Feeding Method in Lactic Acid Production[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2010,162(8):2149-2156.
APA Zhang, Yong,Cong, Wei,Shi, ShaoYuan,&Cong, W.(2010).Application of a pH Feedback-Controlled Substrate Feeding Method in Lactic Acid Production.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,162(8),2149-2156.
MLA Zhang, Yong,et al."Application of a pH Feedback-Controlled Substrate Feeding Method in Lactic Acid Production".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 162.8(2010):2149-2156.
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