| Application of a pH Feedback-Controlled Substrate Feeding Method in Lactic Acid Production | |
| Zhang, Yong1,2; Cong, Wei1,2; Shi, ShaoYuan1,2; Cong, W | |
| 2010-12-01 | |
| Source Publication | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| ISSN | 0273-2289 |
| Volume | 162Issue:8Pages:2149-2156 |
| Abstract | Substrate concentration in lactic acid fermentation broth could not be controlled well by traditional feeding methods, including constant, intermittent, and exponential feeding methods, in fed-batch experiments. A simple feedback feeding method based on pH was proposed to control pH and substrate concentration synchronously to enhance lactic acid production in fed-batch culture. As the linear relationship between the consumption amounts of alkali and that of substrate was concluded during lactic acid fermentation, the alkali and substrate in the feeding broth were mixed together proportionally. Thus, the concentration of substrate could be controlled through the adjustment of pH automatically. In the fed-batch lactic acid fermentation with Lactobacillus lactis-11 by this method, the residual glucose concentration in fermentation broth was controlled between 4.1 and 4.9 g L-1, and the highest concentration of lactic acid, maximum cell dry weight, volumetric productivity of lactic acid, and yield were 96.3 g L-1, 4.7 g L-1, 1.9 g L-1 h(-1) and 0.99 g lactic acid per gram of glucose, respectively, compared to 82.7 g L-1, 3.31 g L-1 1.7 g L-1 h(-1) and 0.92 g lactic acid per gram of glucose in batch culture. This feeding method was simple and easily operated and could be feasible for industrial lactic acid production in the future. |
| Keyword | Lactic Acid Fed-batch Lactobacillus Lactis-11 Ammonium Hydroxide Ph Feedback |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s12010-010-8989-x |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | LACTOBACILLUS-AMYLOPHILUS GV6 ; FED-BATCH CULTURE ; RHIZOPUS-ORYZAE ; SIMULTANEOUS SACCHARIFICATION ; DIRECT FERMENTATION ; AMMONIUM LACTATE ; SUBSP-LACTIS ; STARCH ; BROTH |
| WOS Research Area | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS Subject | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS ID | WOS:000284295000003 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/5411 |
| Collection | 生化工程国家重点实验室 |
| Corresponding Author | Cong, W |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Yong,Cong, Wei,Shi, ShaoYuan,et al. Application of a pH Feedback-Controlled Substrate Feeding Method in Lactic Acid Production[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2010,162(8):2149-2156. |
| APA | Zhang, Yong,Cong, Wei,Shi, ShaoYuan,&Cong, W.(2010).Application of a pH Feedback-Controlled Substrate Feeding Method in Lactic Acid Production.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,162(8),2149-2156. |
| MLA | Zhang, Yong,et al."Application of a pH Feedback-Controlled Substrate Feeding Method in Lactic Acid Production".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 162.8(2010):2149-2156. |
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| 20131111-1086-复件 art（181KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
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