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Magnetic mesoporous silica nanoparticles: Fabrication and their laccase immobilization performance
Wang, Feng; Guo, Chen1; Yang, Liang-rong; Liu, Chun-Zhao1; Guo, C
2010-12-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume101Issue:23Pages:8931-8935
AbstractNewly large-pore magnetic mesoporous silica nanoparticles (MMSNPs) with wormhole framework structures were synthesized for the first time by using tetraethyl orthosilicate as the silica source and amine-terminated Jeffamine surfactants as template. Iminodiacerate was attached on these MMSNPs through a silane-coupling agent and chelated with Cu(2+). The Cu(2+)-chelated MMSNPs (MMSNPs-CPTS-IDA-Cu(2+)) showed higher adsorption capacity of 98.1 mg g(-1)-particles and activity recovery of 92.5% for laccase via metal affinity adsorption in comparison with MMSNPs via physical adsorption. The Michaelis constant (K(m)) and catalytic constant (k(cat)) of laccase immobilized on the MMSNPs-CPTS-IDA-Cu(2+) were 3.28 mM and 155.4 min(-1), respectively. Storage stability and temperature endurance of the immobilized laccase on MMSNPs-CPTS-IDA-Cu(2+) increased significantly, and the immobilized laccase retained 86.6% of its initial activity after 10 successive batch reactions operated with magnetic separation. (C) 2010 Elsevier Ltd. All rights reserved.
KeywordImmobilization Laccase Mesoporous Nanoparticles
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.biortech.2010.06.115
Indexed BySCI
Language英语
WOS KeywordPYCNOPORUS-SANGUINEUS LACCASE ; TRAMETES-VERSICOLOR LACCASE ; CORIOLUS-VERSICOLOR ; PURIFICATION ; ADSORPTION ; BEADS ; DECOLORIZATION ; PARTICLES ; CARRIERS
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000282197200003
Citation statistics
Cited Times:135[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5415
Collection生化工程国家重点实验室
Corresponding AuthorGuo, C
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Feng,Guo, Chen,Yang, Liang-rong,et al. Magnetic mesoporous silica nanoparticles: Fabrication and their laccase immobilization performance[J]. BIORESOURCE TECHNOLOGY,2010,101(23):8931-8935.
APA Wang, Feng,Guo, Chen,Yang, Liang-rong,Liu, Chun-Zhao,&Guo, C.(2010).Magnetic mesoporous silica nanoparticles: Fabrication and their laccase immobilization performance.BIORESOURCE TECHNOLOGY,101(23),8931-8935.
MLA Wang, Feng,et al."Magnetic mesoporous silica nanoparticles: Fabrication and their laccase immobilization performance".BIORESOURCE TECHNOLOGY 101.23(2010):8931-8935.
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