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| Strategies to Improve the Biosynthesis of beta-Lactam Antibiotics by Penicillin G Acylase: Progress and Prospects | |
| Pan, Xin1; Xu, Lei1; Li, Yaru1; Wu, Sihua2,3; Wu, Yong1; Wei, Wenping1 | |
| 2022-07-18 | |
| Source Publication | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
 |
| ISSN | 2296-4185 |
| Volume | 10Pages:14 |
| Abstract | beta-Lactam antibiotics are widely used anti-infection drugs that are traditionally synthesized via a chemical process. In recent years, with the growing demand for green alternatives, scientists have turned to enzymatic synthesis. Penicillin G acylase (PGA) is the second most commercially used enzyme worldwide with both hydrolytic and synthetic activities toward antibiotics, which has been used to manufacture the key antibiotic nucleus on an industrial level. However, the large-scale application of PGA-catalyzed antibiotics biosynthesis is still in the experimental stage because of some key limitations, such as low substrate concentration, unsatisfactory yield, and lack of superior biocatalysts. This paper systematically reviews the strategies adopted to improve the biosynthesis of beta-lactam antibiotics by adjusting the enzymatic property and manipulating the reaction system in recent 20 years, including mining of enzymes, protein engineering, solvent engineering, in situ product removal, and one-pot reaction cascade. These advances will provide important guidelines for the future use of enzymatic synthesis in the industrial production of beta-lactam antibiotics. |
| Keyword | beta-Lactam antibiotics biosynthesis penicillin G acylase enzymatic property reaction system |
| DOI | 10.3389/fbioe.2022.936487 |
| Language | 英语 |
| WOS Keyword | EFFICIENT ENZYMATIC-SYNTHESIS ; HIGH SUBSTRATE CONCENTRATION ; KINETICALLY CONTROLLED SYNTHESIS ; SITE-DIRECTED MUTAGENESIS ; AQUEOUS 2-PHASE SYSTEMS ; CATALYZED SYNTHESIS ; ETHYLENE-GLYCOL ; ACTIVE-SITE ; SYNTHETIC PERFORMANCE ; CEPHALEXIN SYNTHESIS |
| Funding Project | National Natural Science Foundation of China[82000257] ; Natural Science Foundation of Jiangsu Higher Education Institutions of China[20KJB320006] ; Scientific Research Topics of Jiangsu Provincial Health Commission[M2021076] ; Social Development Project of Yangzhou City[YZ2020087] ; Social Development Project of Yangzhou City[YZ2021061] ; Young Talent Support Project of Jiangsu Provincial Association for Science and Technology |
| WOS Research Area | Biotechnology & Applied Microbiology ; Science & Technology - Other Topics |
| WOS Subject | Biotechnology & Applied Microbiology ; Multidisciplinary Sciences |
| Funding Organization | National Natural Science Foundation of China ; Natural Science Foundation of Jiangsu Higher Education Institutions of China ; Scientific Research Topics of Jiangsu Provincial Health Commission ; Social Development Project of Yangzhou City ; Young Talent Support Project of Jiangsu Provincial Association for Science and Technology |
| WOS ID | WOS:000834569200001 |
| Publisher | FRONTIERS MEDIA SA |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/54389 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Pan, Xin; Wu, Yong; Wei, Wenping |
| Affiliation | 1.Yangzhou Univ, Affiliated Hosp Yangzhou Univ, Dept Cardiol, Cent Lab, Yangzhou, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China 3.Gunma Univ, Grad Sch Sci & Technol, Div Mol Sci, Kiryu, Japan |
| Recommended Citation GB/T 7714 | Pan, Xin,Xu, Lei,Li, Yaru,et al. Strategies to Improve the Biosynthesis of beta-Lactam Antibiotics by Penicillin G Acylase: Progress and Prospects[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2022,10:14. |
| APA | Pan, Xin,Xu, Lei,Li, Yaru,Wu, Sihua,Wu, Yong,&Wei, Wenping.(2022).Strategies to Improve the Biosynthesis of beta-Lactam Antibiotics by Penicillin G Acylase: Progress and Prospects.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,10,14. |
| MLA | Pan, Xin,et al."Strategies to Improve the Biosynthesis of beta-Lactam Antibiotics by Penicillin G Acylase: Progress and Prospects".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 10(2022):14. |
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