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| Nanothermometer with Temperature Induced Reversible Emission for Evaluation of Intracellular Thermal Dynamics | |
| Yin, Nana1; Lin, Bo1; Huo, Feng2; Shu, Yang1; Wang, Jianhua1 | |
| 2022-08-24 | |
| Source Publication | ANALYTICAL CHEMISTRY
 |
| ISSN | 0003-2700 |
| Pages | 9 |
| Abstract | Temperature dynamics reflect the physiological state of cells, and accurate measurement of intracellular temperature helps to understand the biological processes. Herein, we report a novel nanothermometer by conjugating a fluorescent probe 3-ethyl-2-[4-(1,2,2-triphenylvinyl)styryl]-benzothiazol-3-ium iodide (TPEBT) with a thermoresponsive polymer poly(N-isopropylacrylamide-co-tetrabutylphosphonium styrenesulfonate) [P(NIPAM-co-TPSS)]. The derived nanoprobe TPEBT-P(NIPAM-co-TPSS) self-assembles into micelles with TPEBT as hydrophobic core and PNIPAM as hydrophilic shell. It exhibits aggregation-induced emission (AIE) at lambda(ex)/lambda(em) = 420/640 nm in aqueous medium with a quantum yield of phi(F) 11.9%. The rise in temperature transforms PNIPAM chains from linear to compact spheres to serve as the core of micelles, and meanwhile converts TPEBT from the state of aggregation to dispersion and redistributes in the micellar shell. Temperature driven phase transition of P(NIPAM-co-TPSS) mediates the reversible aggregation and disaggregation of TPEBT and endows the nanothermometer with temperature-dependent AIE features and favorable sensitivity for temperature sensing in 32-40 ?. TPEBT-P(NIPAM-co-TPSS) is taken up by HeLa cells to distribute mainly in lysosomes. It enables quantitative visualization of in situ thermal dynamics in response to stimuli from carbonyl cyanide 4(trifluoromethoxy)phenylhydrazone, oligomycin, genipin, and lipopolysaccharide. The real-time monitoring of photothermal-induced intracellular temperature variation is further conducted. |
| DOI | 10.1021/acs.analchem.2c02106 |
| Language | 英语 |
| Funding Project | Natural Science Foundation of China[21974018] ; Natural Science Foundation of China[22174013] ; Natural Science Foundation of China[22074011] |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Analytical |
| Funding Organization | Natural Science Foundation of China |
| WOS ID | WOS:000848534500001 |
| Publisher | AMER CHEMICAL SOC |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/54540 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Shu, Yang; Wang, Jianhua |
| Affiliation | 1.Northeastern Univ, Coll Sci, Dept Chem, Shenyang 110819, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing Key Lab Ion Liquids Clean Proc, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Yin, Nana,Lin, Bo,Huo, Feng,et al. Nanothermometer with Temperature Induced Reversible Emission for Evaluation of Intracellular Thermal Dynamics[J]. ANALYTICAL CHEMISTRY,2022:9. |
| APA | Yin, Nana,Lin, Bo,Huo, Feng,Shu, Yang,&Wang, Jianhua.(2022).Nanothermometer with Temperature Induced Reversible Emission for Evaluation of Intracellular Thermal Dynamics.ANALYTICAL CHEMISTRY,9. |
| MLA | Yin, Nana,et al."Nanothermometer with Temperature Induced Reversible Emission for Evaluation of Intracellular Thermal Dynamics".ANALYTICAL CHEMISTRY (2022):9. |
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