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红平红球菌LSSE8-1脱硫代谢及其相关脱硫基因的比较
缑仲轩; 罗明芳; 李信; 邢建民; 刘会洲
2003
Source Publication科学通报
Issue22Pages:2343-2349
Abstract红平红球菌(Rhodococcus erythropolis)LSSE8-1是从我国贵州赤水气田中筛选分离的一株专一性脱硫菌.该菌能选择性地氧化二苯并噻吩(DBT)生成2-羟基联苯(HBP).利用DBTO_2(dibenzothiophene5,5-dioxide)作底物既生成HBP又生成DBT,表明从DBT到DBTO_2这一步反应在细胞水平是可逆的.同时以DBT为对照,0.01~0.4 mmol/L DBTO_2均对细胞的生长表现出毒害作用,这就解释了脱硫反应中DBTO_2不积累的问题.在溶菌酶处理的基础上,用碱液裂解法提取LSSE8-1质粒作模板,选择Rhodococcus erythropolis IGTS8脱硫基因的保守区设计引物,用PCR的方法扩增分别得到 1.3,1.0和1.2 kb三段脱硫基因片段.选用pGEM-T easy载体,E.coli.DH5α为受体菌,克隆这三段基因后,测序结果表明这三段脱硫基因高度保守.与IGTS8的相关脱硫基因相比较,dszA和dszB的同源性都是100%,dszC的同源性是99%.
Keyword二苯并噻吩 四硫途径 中间产物 基因序列 脱硫 红平红球菌
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5460
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
缑仲轩,罗明芳,李信,等. 红平红球菌LSSE8-1脱硫代谢及其相关脱硫基因的比较[J]. 科学通报,2003(22):2343-2349.
APA 缑仲轩,罗明芳,李信,邢建民,&刘会洲.(2003).红平红球菌LSSE8-1脱硫代谢及其相关脱硫基因的比较.科学通报(22),2343-2349.
MLA 缑仲轩,et al."红平红球菌LSSE8-1脱硫代谢及其相关脱硫基因的比较".科学通报 .22(2003):2343-2349.
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