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| Engineering synthetic microbial consortium for cadaverine biosynthesis from glycerol | |
| Liu, Simin1,2,3; Mi, Jiali1,2,3; Song, Kejing4; Qi, Haishan1,2,3; Zhang, Lei1,2,3 | |
| 2022-10-06 | |
| Source Publication | BIOTECHNOLOGY LETTERS
 |
| ISSN | 0141-5492 |
| Pages | 12 |
| Abstract | Objectives 1,5-pentanediamine (cadaverine) is a C5 platform chemical, also an important raw material for bio-polyamide PA5X. With increasing concerns about the depletion of fossil resources and global environmental protection, cadaverine bio-production has attracted more attentions. Results Here, a microbial consortium consisting of Corynebacterium glutamicum cgl-FDK and Escherichia coli BL-ABST-Spy was constructed to de novo synthesize cadaverine utilizing glycerol as the sole carbon resource. The glycerol utilization pathway was initially constructed in C. glutamicum cgl-FDK to produce lysine from glycerol. Then, the pyridoxal 5 '-phosphate (PLP) biosynthesis pathway and SpyTag/SpyCatcher protein-ligation system for lysine decarboxylase (CadA) and cadaverine-lysine antiporter protein (CadB) were introduced into E. coli BL-ABST-Spy to synthesize cadaverine from lysine. Furthermore, the fermentation conditions of microbial consortium were optimized and the cadaverine production reached 9.3 g/L with glycerol as the sole carbon source. Conclusions This work provides a promising strategy for efficiently producing cadaverine from glycerol with an artificial microbial consortium. |
| Keyword | Cadaverine De novo synthesis Glycerol Microbial consortium Synthetic biology |
| DOI | 10.1007/s10529-022-03306-2 |
| Language | 英语 |
| WOS Keyword | CORYNEBACTERIUM-GLUTAMICUM ; ESCHERICHIA-COLI ; PATHWAY ; STRAIN |
| Funding Project | National Key R&D Program of China[2018YFA0902200] ; National Natural Science Foundation of China[21606165] ; National Natural Science Foundation of China[21621004] ; National Natural Science Foundation of China[21422605] ; Program of Introducing Talents of Discipline to Universities[BP0618007] |
| WOS Research Area | Biotechnology & Applied Microbiology |
| WOS Subject | Biotechnology & Applied Microbiology |
| Funding Organization | National Key R&D Program of China ; National Natural Science Foundation of China ; Program of Introducing Talents of Discipline to Universities |
| WOS ID | WOS:000864597800002 |
| Publisher | SPRINGER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/55037 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Qi, Haishan |
| Affiliation | 1.Tianjin Univ, Sch Chem Engn & Technol, Dept Biochem Engn, 135 Yaguan Rd, Tianjin 300072, Peoples R China 2.Tianjin Univ, Sch Chem Engn & Technol, Frontier Sci Ctr Synthet Biol, 135 Yaguan Rd, Tianjin 300350, Peoples R China 3.Tianjin Univ, Key Lab Syst Bioengn MOE, 135 Yaguan Rd, Tianjin 300350, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Liu, Simin,Mi, Jiali,Song, Kejing,et al. Engineering synthetic microbial consortium for cadaverine biosynthesis from glycerol[J]. BIOTECHNOLOGY LETTERS,2022:12. |
| APA | Liu, Simin,Mi, Jiali,Song, Kejing,Qi, Haishan,&Zhang, Lei.(2022).Engineering synthetic microbial consortium for cadaverine biosynthesis from glycerol.BIOTECHNOLOGY LETTERS,12. |
| MLA | Liu, Simin,et al."Engineering synthetic microbial consortium for cadaverine biosynthesis from glycerol".BIOTECHNOLOGY LETTERS (2022):12. |
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