Knowledge Management System Of Institute of process engineering,CAS
| Enzyme-driven oxygen-fuelled pathway selectivity of tyrosine-containing peptide oxidation evolution | |
| Ren, Xiaokang1,2; Zhao, Luyang1; Yuan, Chengqian1; Shi, Mengqian1; Xing, Ruirui1,2; Yan, Xuehai1,2,3 | |
| 2022-12-15 | |
| Source Publication | CHEMICAL ENGINEERING JOURNAL
 |
| ISSN | 1385-8947 |
| Volume | 450Pages:8 |
| Abstract | The pathway selectivity of chemical evolution controlled by enzymes, with proteins and peptides as substrates, is of critical significance to determine biological processes and functions. In nature, organisms utilize tyrosinase to catalyse oxidation reactions of tyrosine to produce multifunctional compounds in different pathways. However, it remains a great challenge to manipulate the pathway selection of tyrosinase-guided chemical evolution for achieving biomaterials with on-demand functions. We herein report that controlling oxygen concentration is effective in regulating the evolution of tyrosinase-catalysed oxygenation pathways of tyrosine-containing peptides and even proteins. The melanin formation pathway predominates under oxygen-enriched conditions, while fluorescent compounds are preferentially generated under hypoxic conditions. The chemical evolution of tyrosine-containing peptides, regardless of their neighbouring amino acid types and sequences, tends to be governed by these two competitive pathways. These competitive pathways are manipulated by conformational transformation of tyrosinase active centre that is determined by oxygen concentration. Moreover, the oxygen-controlled chemical evolution of tyrosine-containing biomolecules can be broadly realized by tyrosinase to obtain melanin-based photothermal materials or multicolour fluorescent materials. This work reveals the plausible role of oxygen in the pathway selectivity of enzyme-controlled chemical evolution of tyrosine-containing peptides and verifies the possibility of fluorescent detection in living cultured melanoma cells, which provides a versatile strategy for precise design and engineering of tyrosine-involved multifunctional biomaterials. |
| Keyword | Peptide Tyrosine Enzymatic oxygenation Chemical evolution Pathway selectivity |
| DOI | 10.1016/j.cej.2022.138293 |
| Language | 英语 |
| WOS Keyword | MELANIN ; RED |
| Funding Project | National Science Fund for Distinguished Young Scholars of China[22025207] ; Innovation Research Community Science Fund of China[21821005] ; National Natural Science Foundation of China[22072154] ; National Natural Science Foundation of China[22077122] ; National Natural Science Foundation of Hebei Province[B2020103025] ; National Natural Science Foundation of Hebei Province[B2020103036] ; Youth Innovation Promotion Association of Chinese Academy of Sciences[2021048] |
| WOS Research Area | Engineering |
| WOS Subject | Engineering, Environmental ; Engineering, Chemical |
| Funding Organization | National Science Fund for Distinguished Young Scholars of China ; Innovation Research Community Science Fund of China ; National Natural Science Foundation of China ; National Natural Science Foundation of Hebei Province ; Youth Innovation Promotion Association of Chinese Academy of Sciences |
| WOS ID | WOS:000875249700005 |
| Publisher | ELSEVIER SCIENCE SA |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/55300 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Xing, Ruirui; Yan, Xuehai |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, 1 North Second St, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Ctr Mesosci, 1 North Second St, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Ren, Xiaokang,Zhao, Luyang,Yuan, Chengqian,et al. Enzyme-driven oxygen-fuelled pathway selectivity of tyrosine-containing peptide oxidation evolution[J]. CHEMICAL ENGINEERING JOURNAL,2022,450:8. |
| APA | Ren, Xiaokang,Zhao, Luyang,Yuan, Chengqian,Shi, Mengqian,Xing, Ruirui,&Yan, Xuehai.(2022).Enzyme-driven oxygen-fuelled pathway selectivity of tyrosine-containing peptide oxidation evolution.CHEMICAL ENGINEERING JOURNAL,450,8. |
| MLA | Ren, Xiaokang,et al."Enzyme-driven oxygen-fuelled pathway selectivity of tyrosine-containing peptide oxidation evolution".CHEMICAL ENGINEERING JOURNAL 450(2022):8. |
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