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Methoxy poly (ethylene glycol) thiosulfonate: new activated polymer derivatives for thiol-specific modification
Zhao, Yong-Jiang1,2; Zhai, Yan-qin1,2; Su, Zhi-Guo1; Ma, Guang-Hui1; Ma, GH
2010-12-01
Source PublicationPOLYMERS FOR ADVANCED TECHNOLOGIES
ISSN1042-7147
Volume21Issue:12Pages:867-873
AbstractThiol-reactive methoxy poly (ethylene glycol) (mPEG) derivatives, mPEG methanethiosulfonate (mPEG-MTS) and mPEG phenylthiosulfonate (mPEG-PTS), were synthesized by one-step S-alkylation of mPEG-halide with the corresponding sodium thiosulfonate. These functionalized polymers were fully characterized by H-1 NMR, C-13 NMR, GPC as well as MALDI-TOF. To demonstrate the potential application of mPEG-MTS and mPEG-PTS, papain and recombinant human granulocyte colony-stimulating factor (rhG-CSF) were used as prototype protein for thiol-selective PEGylation. The PEGylated products were verified by one or more of the following methods: SDS-PAGE, HPLC, HPSEC as well as MALDI-TOF. Compared with native rhG-CSF, the PEGylated rhG-CSF was further characterized by in vitro cell proliferative assay and preserved 59% biological activity. Furthermore, mPEG20k-MTS was used to modify BSA nanoparticles coated with thiol groups. FT-IR and zeta-potential assay demonstrated that mPEG thiosulfonate can be effectively immobilized at the nanoparticles' surface via disulfide linkage to form hydrophilic and neutral corona. Therefore, we have demonstrated thiol-selective PEG derivatives, endowed with unusual versatility, for practical use and potential exploitation. Copyright (C) 2009 John Wiley & Sons, Ltd.
KeywordConjugated Polymers Cysteine-selective Pegylation Drug Delivery System Functionalization Of Polymers Mpeg Thiosulfonate
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1002/pat.1512
Indexed BySCI
Language英语
WOS KeywordSITE-SPECIFIC PEGYLATION ; COLONY-STIMULATING FACTOR ; POLYETHYLENE-GLYCOL ; POLY(ETHYLENE GLYCOL) ; PROTEIN MODIFICATION ; REAGENTS ; GLYCOSYLATION ; HEMOGLOBIN ; DELIVERY ; PEPTIDE
WOS Research AreaPolymer Science
WOS SubjectPolymer Science
WOS IDWOS:000285431700005
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5564
Collection生化工程国家重点实验室
Corresponding AuthorMa, GH
Affiliation1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Yong-Jiang,Zhai, Yan-qin,Su, Zhi-Guo,et al. Methoxy poly (ethylene glycol) thiosulfonate: new activated polymer derivatives for thiol-specific modification[J]. POLYMERS FOR ADVANCED TECHNOLOGIES,2010,21(12):867-873.
APA Zhao, Yong-Jiang,Zhai, Yan-qin,Su, Zhi-Guo,Ma, Guang-Hui,&Ma, GH.(2010).Methoxy poly (ethylene glycol) thiosulfonate: new activated polymer derivatives for thiol-specific modification.POLYMERS FOR ADVANCED TECHNOLOGIES,21(12),867-873.
MLA Zhao, Yong-Jiang,et al."Methoxy poly (ethylene glycol) thiosulfonate: new activated polymer derivatives for thiol-specific modification".POLYMERS FOR ADVANCED TECHNOLOGIES 21.12(2010):867-873.
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