CAS OpenIR  > 生化工程国家重点实验室
Regulation of protein multipoint adsorption on ion-exchange adsorbent and its application to the purification of macromolecules
Huang, Yongdong1; Bi, Jingxiu1,2; Zhao, Lan1,3; Ma, Guanghui1; Su, Zhiguo1; Su, ZG
2010-12-01
Source PublicationPROTEIN EXPRESSION AND PURIFICATION
ISSN1046-5928
Volume74Issue:2Pages:257-263
AbstractIon-exchange chromatography (IEC) using commercial ionic absorbents is a widely used technique for protein purification. Protein adsorption onto ion-exchange adsorbents often involves a multipoint adsorption. In IEC of multimeric proteins or "soft" proteins, the intense multipoint binding would make the further desorption difficult, even lead to the destruction of protein structure and the loss of its biological activity. In this paper, DEAE Sepharose FF adsorbents with controllable ligand densities from 0.020 to 0.183 mmol/ml were synthesized, and then the effect of ligand density on the static ion-exchange adsorption of bovine serum albumin (BSA) onto DEAE Sepharose FF was studied by batch adsorption technique. Steric mass-action (SMA) model was employed to analyze the static adsorption behavior. The results showed that the SMA model parameters, equilibrium constant (K(a)), characteristic number of binding sites (upsilon) and steric factor (sigma), increased gradually with ligand density. Thus, it was feasible to regulate BSA multipoint adsorption by modulating the ligand density of ion-exchange adsorbent. Furthermore, IEC of hepatitis B surface antigen (HBsAg) using DEAE Sepharose FF adsorbents with different ligand densities was carried out, and the activity recovery of HBsAg was improved from 42% to 67% when the ligand density was decreased from 0.183 to 0.020 mmol/ml. Taking the activity recovery of HBsAg, the purification factor and the binding capacity into account, DEAE Sepharose FF with a ligand density of 0.041 mmol/ml was most effective for the purification of HBsAg. Such a strategy may also be beneficial for the purification of macromolecules and multimeric proteins. (C) 2010 Elsevier Inc. All rights reserved.
KeywordMultipoint Adsorption Ligand Density Steric Mass-action Model Bovine Serum Albumin Hepatitis b Surface Antigen Macromolecule
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.pep.2010.07.002
Indexed BySCI
Language英语
WOS KeywordVIRUS SURFACE-ANTIGEN ; HYDROPHOBIC INTERACTION CHROMATOGRAPHY ; SIZE-EXCLUSION CHROMATOGRAPHY ; CHINESE-HAMSTER OVARY ; BOVINE SERUM-ALBUMIN ; MASS-ACTION MODEL ; LIGAND DENSITY ; AFFINITY-CHROMATOGRAPHY ; DISTRIBUTIONS ; EQUILIBRIUM
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000283206700018
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5565
Collection生化工程国家重点实验室
Corresponding AuthorSu, ZG
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Adelaide, Sch Pharmaceut Engn, Adelaide, SA 5005, Australia
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Huang, Yongdong,Bi, Jingxiu,Zhao, Lan,et al. Regulation of protein multipoint adsorption on ion-exchange adsorbent and its application to the purification of macromolecules[J]. PROTEIN EXPRESSION AND PURIFICATION,2010,74(2):257-263.
APA Huang, Yongdong,Bi, Jingxiu,Zhao, Lan,Ma, Guanghui,Su, Zhiguo,&Su, ZG.(2010).Regulation of protein multipoint adsorption on ion-exchange adsorbent and its application to the purification of macromolecules.PROTEIN EXPRESSION AND PURIFICATION,74(2),257-263.
MLA Huang, Yongdong,et al."Regulation of protein multipoint adsorption on ion-exchange adsorbent and its application to the purification of macromolecules".PROTEIN EXPRESSION AND PURIFICATION 74.2(2010):257-263.
Files in This Item:
File Name/Size DocType Version Access License
20131112-1101-复件 1-s(613KB) 限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Huang, Yongdong]'s Articles
[Bi, Jingxiu]'s Articles
[Zhao, Lan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Huang, Yongdong]'s Articles
[Bi, Jingxiu]'s Articles
[Zhao, Lan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Huang, Yongdong]'s Articles
[Bi, Jingxiu]'s Articles
[Zhao, Lan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.