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Preparation and characterization of a bovine serum albumin-catalase conjugate
Alternative TitleBiotechnol. Lett.
Hu, T; Su, ZG
2002-06-01
Source PublicationBIOTECHNOLOGY LETTERS
ISSN0141-5492
Volume24Issue:12Pages:1027-1030
AbstractA bovine serum albumin-catalase conjugate was prepared using a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The purified conjugate had an M-r of 380 kDa. The half-life time of the conjugate was 6, 69 and 65 min after heat treatment, urea treatment and trypsin hydrolysis, respectively, whereas that of native catalase was 3, 22, 28 min, respectively.; A bovine serum albumin-catalase conjugate was prepared using a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The purified conjugate had an M-r of 380 kDa. The half-life time of the conjugate was 6, 69 and 65 min after heat treatment, urea treatment and trypsin hydrolysis, respectively, whereas that of native catalase was 3, 22, 28 min, respectively.
KeywordBovine Serum Albumin Catalase O-2 Free Radicals N-succinimidyl 3-(2-pyridyldithio)Propionate
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
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Indexed BySCI
Language英语
WOS KeywordSUPEROXIDE-DISMUTASE ; INJURY
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000175806500014
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5600
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Hu, T,Su, ZG. Preparation and characterization of a bovine serum albumin-catalase conjugate[J]. BIOTECHNOLOGY LETTERS,2002,24(12):1027-1030.
APA Hu, T,&Su, ZG.(2002).Preparation and characterization of a bovine serum albumin-catalase conjugate.BIOTECHNOLOGY LETTERS,24(12),1027-1030.
MLA Hu, T,et al."Preparation and characterization of a bovine serum albumin-catalase conjugate".BIOTECHNOLOGY LETTERS 24.12(2002):1027-1030.
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