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Preparation of well-defined bovine polyhemoglobin based on dimethyl adipimidate and glutaraldebyde cross-linkage
Alternative TitleBiochem. Biophys. Res. Commun.
Hu, T; Su, ZG
2002-05-10
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
Volume293Issue:3Pages:958-961
AbstractA well-defined bovine polyhemoglobin was prepared by dimethyl adipimidate (DMA) and glutaraldehyde double cross-linkage method. DMA was used to block some amino groups of hemoglobin, followed by further polymerization with glutaraldehyde. The amino modification degree of hemoglobin was 32% when DMA reacted with hemoglobin at the molar ratio of 200. The bovine polyhemoglobin with narrow molecular weight distribution (mainly 128 kDa) was obtained when glutaraldehyde reacted with DMA-modified hemoglobin. The P-50 and the Hill coefficient for DMA-modified hemoglobin were 19.4 mm Hg and 2.28, respectively, while those for the bovine polyhemoglobin were 15.1 mm Hg and 1.70, respectively. The number of Bohr protons released for DMA-modified hemoglobin and the poly hemoglobin was 0.86 and 0.56 H/tetramer, respectively. (C) 2002 Elsevier Science (USA). All rights reserved.; A well-defined bovine polyhemoglobin was prepared by dimethyl adipimidate (DMA) and glutaraldehyde double cross-linkage method. DMA was used to block some amino groups of hemoglobin, followed by further polymerization with glutaraldehyde. The amino modification degree of hemoglobin was 32% when DMA reacted with hemoglobin at the molar ratio of 200. The bovine polyhemoglobin with narrow molecular weight distribution (mainly 128 kDa) was obtained when glutaraldehyde reacted with DMA-modified hemoglobin. The P-50 and the Hill coefficient for DMA-modified hemoglobin were 19.4 mm Hg and 2.28, respectively, while those for the bovine polyhemoglobin were 15.1 mm Hg and 1.70, respectively. The number of Bohr protons released for DMA-modified hemoglobin and the poly hemoglobin was 0.86 and 0.56 H/tetramer, respectively. (C) 2002 Elsevier Science (USA). All rights reserved.
KeywordGlutaraldehyde Dimethyl Adipimidate Polyhemoglobin Cross-linkage Blood Substitute
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
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Indexed BySCI
Language英语
WOS KeywordHEMOGLOBIN ; BLOOD ; POLYMERIZATION
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000175640800012
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Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5601
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Hu, T,Su, ZG. Preparation of well-defined bovine polyhemoglobin based on dimethyl adipimidate and glutaraldebyde cross-linkage[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,293(3):958-961.
APA Hu, T,&Su, ZG.(2002).Preparation of well-defined bovine polyhemoglobin based on dimethyl adipimidate and glutaraldebyde cross-linkage.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,293(3),958-961.
MLA Hu, T,et al."Preparation of well-defined bovine polyhemoglobin based on dimethyl adipimidate and glutaraldebyde cross-linkage".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 293.3(2002):958-961.
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