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| Study on ionic liquids based novel method for separation and purification of silkworm pupa protein | |
| Zhang, Wen1,2,4; Yang, Shaoqi1,3; Ren, Baozeng4; Lu, Xingmei1; Jia, Cai1 | |
| 2022 | |
| Source Publication | CHINESE CHEMICAL LETTERS
 |
| ISSN | 1001-8417 |
| Volume | 34Issue:1Pages:6 |
| Abstract | Silkworm pupa protein (SPP) that obtained by traditional method usually had a high fat content, which would impose restrictions on the further use of SPP. Herein, various functionalized ionic liquids (ILs) were used to extract SPP from silkworm pupae, the structure-performance relationship of ILs with their SPP separation performance were explored at the same time. The research showed that the maxi-mum extraction yield of SPP was up to 62.6% with less than 0.5% low fat content by using 1-ethyl-3-methylimidazolium chloride ([Emim]Cl), when the dissolution experiment was conducted at 90 ? for 24 h with ethanol bath as the regeneration solvent. Comparing with the structure of raw material, the regenerated SPP maintained the native protein backbone. Meanwhile, all regenerated SPP showed a de-creased crystallinity, which also exhibited decreased fraction of the alpha-helix comparing to that beta-sheet united with coil random structures. (C) 2022 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. |
| Keyword | Silkworm pupa protein Ionic liquids Protein backbone Silkworm pupa Separation |
| DOI | 10.1016/j.cclet.2022.04.072 |
| Language | 英语 |
| WOS Keyword | WOOL KERATIN ; CELLULOSE ; DISSOLUTION ; EXTRACTION |
| Funding Project | National Natural Science Foundation of China[81673400] ; National Natural Science Foundation of China[21878292] |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Multidisciplinary |
| Funding Organization | National Natural Science Foundation of China |
| WOS ID | WOS:000893634100025 |
| Publisher | ELSEVIER SCIENCE INC |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/56137 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Jia, Cai |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, Beijing Key Lab Ion Liquids Clean Proc, CAS Key Lab Green Proc & Engn,State Key Lab Multip, Beijing 100190, Peoples R China 2.Zhengzhou Inst Emerging Ind Technol, Zhengzhou 450000, Peoples R China 3.Henan Agr Univ, Coll Mech & Elect Engn, Key Lab New Mat & Facil Rural Renewable Energy, Minist Agr & Rural Affairs, Zhengzhou 450002, Peoples R China 4.Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Wen,Yang, Shaoqi,Ren, Baozeng,et al. Study on ionic liquids based novel method for separation and purification of silkworm pupa protein[J]. CHINESE CHEMICAL LETTERS,2022,34(1):6. |
| APA | Zhang, Wen,Yang, Shaoqi,Ren, Baozeng,Lu, Xingmei,&Jia, Cai.(2022).Study on ionic liquids based novel method for separation and purification of silkworm pupa protein.CHINESE CHEMICAL LETTERS,34(1),6. |
| MLA | Zhang, Wen,et al."Study on ionic liquids based novel method for separation and purification of silkworm pupa protein".CHINESE CHEMICAL LETTERS 34.1(2022):6. |
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