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Refolding of superoxide dismutase by ion-exchange chromatography
Alternative TitleBiotechnol. Lett.
Li, M; Su, ZG
2002-06-01
Source PublicationBIOTECHNOLOGY LETTERS
ISSN0141-5492
Volume24Issue:11Pages:919-923
AbstractA new ion-exchange chromatography process was developed for refolding of iron superoxide dismutase (Fe-SOD) produced in Escherichia coli as an inclusion body. After adsorption on an ion-exchange matrix, the denatured protein was eluted by gradient decrease of urea concentration and pH of the elution buffer. The dual gradient allowed the denatured protein to refold to its correct native conformation with return of biological activity. Compared with the traditional dilution, refolding process, the new process increased the refolding yield five-fold. The process could also be carried out at high protein concentration to decrease the solution volume after refolding.; A new ion-exchange chromatography process was developed for refolding of iron superoxide dismutase (Fe-SOD) produced in Escherichia coli as an inclusion body. After adsorption on an ion-exchange matrix, the denatured protein was eluted by gradient decrease of urea concentration and pH of the elution buffer. The dual gradient allowed the denatured protein to refold to its correct native conformation with return of biological activity. Compared with the traditional dilution, refolding process, the new process increased the refolding yield five-fold. The process could also be carried out at high protein concentration to decrease the solution volume after refolding.
KeywordGradient Ion-exchange Chromatography Inclusion Body Ph Refolding Superoxide Dismutase Urea Concentration
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordESCHERICHIA-COLI ; LYSOZYME ; RENATURATION ; PROTEINS ; YIELD
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000175752100012
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5614
Collection研究所(批量导入)
AffiliationChinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100864, Peoples R China
Recommended Citation
GB/T 7714
Li, M,Su, ZG. Refolding of superoxide dismutase by ion-exchange chromatography[J]. BIOTECHNOLOGY LETTERS,2002,24(11):919-923.
APA Li, M,&Su, ZG.(2002).Refolding of superoxide dismutase by ion-exchange chromatography.BIOTECHNOLOGY LETTERS,24(11),919-923.
MLA Li, M,et al."Refolding of superoxide dismutase by ion-exchange chromatography".BIOTECHNOLOGY LETTERS 24.11(2002):919-923.
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