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Dual gradient ion-exchange chromatography improved refolding yield of lysozyme
Alternative TitleJ. Chromatogr. A
Li, M; Zhang, GF; Su, ZG
2002-06-14
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume959Issue:1-2Pages:113-120
AbstractProtein refolding at high concentrations always leads to aggregation, which limits commercial application. An ion-exchange chromatography process with gradient changes in urea concentration and pH was developed to refold denatured lysozyme at high concentration. After adsorption of the denatured protein onto an ion-exchange medium, elution was carried out in combination with a gentle decrease in urea concentration and elevation of pH. Protein would gradually refold along the column with high activity yield. Denatured and reduced lysozyme at 40 mg/ml was loaded into a column filled with SP Sepharose Fast Flow, resulting in 95% activity recovery and 98% mass yield within a short period of time. (C) 2002 Elsevier Science B.V. All rights reserved.; Protein refolding at high concentrations always leads to aggregation, which limits commercial application. An ion-exchange chromatography process with gradient changes in urea concentration and pH was developed to refold denatured lysozyme at high concentration. After adsorption of the denatured protein onto an ion-exchange medium, elution was carried out in combination with a gentle decrease in urea concentration and elevation of pH. Protein would gradually refold along the column with high activity yield. Denatured and reduced lysozyme at 40 mg/ml was loaded into a column filled with SP Sepharose Fast Flow, resulting in 95% activity recovery and 98% mass yield within a short period of time. (C) 2002 Elsevier Science B.V. All rights reserved.
KeywordGradient Elution Protein Folding Ph Effects Lysozyme Proteins Urea
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
URL查看原文
Indexed BySCI
Language英语
WOS KeywordSIZE-EXCLUSION CHROMATOGRAPHY ; IMMOBILIZED LIPOSOME CHROMATOGRAPHY ; EGG-WHITE LYSOZYME ; ESCHERICHIA-COLI ; INCLUSION-BODIES ; OXIDATIVE RENATURATION ; CARBONIC-ANHYDRASE ; REDUCED LYSOZYME ; PROTEIN ; PURIFICATION
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000176608400011
Citation statistics
Cited Times:72[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5615
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Li, M,Zhang, GF,Su, ZG. Dual gradient ion-exchange chromatography improved refolding yield of lysozyme[J]. JOURNAL OF CHROMATOGRAPHY A,2002,959(1-2):113-120.
APA Li, M,Zhang, GF,&Su, ZG.(2002).Dual gradient ion-exchange chromatography improved refolding yield of lysozyme.JOURNAL OF CHROMATOGRAPHY A,959(1-2),113-120.
MLA Li, M,et al."Dual gradient ion-exchange chromatography improved refolding yield of lysozyme".JOURNAL OF CHROMATOGRAPHY A 959.1-2(2002):113-120.
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