Straw lignin degradation by lignin peroxidase from Irpex lacteus cooperated with enzymes and small molecules

doi:10.1007/s10529-022-03325-z

CAS OpenIR

	Straw lignin degradation by lignin peroxidase from Irpex lacteus cooperated with enzymes and small molecules
	Chen, Huayou 1,2; Liang, Xiaoyu 1; Li, Shouzhi 1; Cui, Zhoulei 1; Yong, Yangchun 1; Ni, Zhong 1; Bu, Quan 1; Zhu, Daochen 1
	2022-12-09
Source Publication	BIOTECHNOLOGY LETTERS
ISSN	0141-5492
Pages	10
Abstract	Objectives Maximizing the utility value of enzymes was achieved by exploring the effects of small molecules on the efficiency of lignin degradation by lignin peroxidase. Methods Using wheat straw as raw material and taking lignin degradation rate as index, it was found that laccase, glucose oxidase, malonic acid, citric acid, ZnSO4, CaCl2 could promote the lignin degradation by the lignin peroxidase from Irpex lacteus, respectively. Moreover, glucose oxidase, malonic acid and CaCl2 had obvious synergy effects on lignin degradation by the lignin peroxidase. Results The optimal conditions of lignin degradation were obtained by response surface experiment: 4% glucose oxidase, 0.74% malonic acid and 3.29% CaCl2 were added for synergistic degradation at 37 degrees C with 50% of water content. After 72 h quickly enzymatic hydrolysis, the degradation rate of lignin was 45.84%. Conclusions A new green and efficient method for lignin removal from straw was obtained, which provided a reference for the efficient utilization of straw and lignin peroxidase.
Keyword	CaCl2 Degradation Glucose oxidase Lignin Malonic acid
DOI	10.1007/s10529-022-03325-z
Language	英语
WOS Keyword	PHANEROCHAETE-CHRYSOSPORIUM ; BIODEGRADATION
Funding Project	National Key Research and Development Program[2021YFA0910402] ; National Key Research and Development Program[2021YFC2103004] ; Open Funding Project of the State Key Laboratory of Biochemical Engineering, China[2018KF-02]
WOS Research Area	Biotechnology & Applied Microbiology
WOS Subject	Biotechnology & Applied Microbiology
Funding Organization	National Key Research and Development Program ; Open Funding Project of the State Key Laboratory of Biochemical Engineering, China
WOS ID	WOS:000895589800002
Publisher	SPRINGER
Citation statistics
Document Type	期刊论文
Identifier	http://ir.ipe.ac.cn/handle/122111/56238
Collection	中国科学院过程工程研究所
Corresponding Author	Chen, Huayou
Affiliation	1.Jiangsu Univ, Sch Life Sci, Zhenjiang 212013, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 10090, Peoples R China
Recommended Citation GB/T 7714	Chen, Huayou,Liang, Xiaoyu,Li, Shouzhi,et al. Straw lignin degradation by lignin peroxidase from Irpex lacteus cooperated with enzymes and small molecules[J]. BIOTECHNOLOGY LETTERS,2022:10.
APA	Chen, Huayou.,Liang, Xiaoyu.,Li, Shouzhi.,Cui, Zhoulei.,Yong, Yangchun.,...&Zhu, Daochen.(2022).Straw lignin degradation by lignin peroxidase from Irpex lacteus cooperated with enzymes and small molecules.BIOTECHNOLOGY LETTERS,10.
MLA	Chen, Huayou,et al."Straw lignin degradation by lignin peroxidase from Irpex lacteus cooperated with enzymes and small molecules".BIOTECHNOLOGY LETTERS (2022):10.

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