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Effect of Amorphophallus Konjac oligosaccharides on STZ-induced diabetes model of isolated islets
Alternative TitleLife Sci.
Lu, XJ; Chen, XM; Fu, DX; Cong, W; Fan, OY
2002-12-27
Source PublicationLIFE SCIENCES
ISSN0024-3205
Volume72Issue:6Pages:711-719
AbstractThree oligosaccharide fractions from the root of Amorphophallus Konjac, which was reported with hypoglycemic effects on diabetes subjects, were isolated and studied using the STZ-treated diabetes model. Among them, one fraction named as KOS-A, was found with nitric oxide (NO.) free radical regulation effect, while the other two were not. At concentrations less than 1.5 mM, KOS-A positively decreased STZ-induced NO. level of islets, but normal NO. release for non-STZ-treated islets was not affected within the range. At 15 film, KOS-A played a contrary role and increased NO. level for islets both with and without STZ-treatment. Islets insulin secretion changed corresponding to NO. level in the assay. Increased insulin secretion appeared parallel to the decrease of NO., and normal insulin release was not affected by KOS-A less than 1.5 mM. Structure determination of KOS-A shows that it is a tetrasaccharide with Mw of 666 Da and reductive end of alpha-D-mannose. These results indicate that low dosage of KOS-A, with its function on attenuating STZ-induced NO. level, doesn't alter normal NO. and insulin secretion pathways of isolated islets. The NO. attenuation function of KOS-A on the diabetes model is mainly resulted from environmental free radical scavenging by the oligosaccharide. Present results also imply the mechanism of clinical Amorphophallus Konjac hypoglycemic function maybe related with free radical attenuation and lower risks of islets damage from NO. radical. (C) 2002 Elsevier Science Inc. All rights reserved.; Three oligosaccharide fractions from the root of Amorphophallus Konjac, which was reported with hypoglycemic effects on diabetes subjects, were isolated and studied using the STZ-treated diabetes model. Among them, one fraction named as KOS-A, was found with nitric oxide (NO.) free radical regulation effect, while the other two were not. At concentrations less than 1.5 mM, KOS-A positively decreased STZ-induced NO. level of islets, but normal NO. release for non-STZ-treated islets was not affected within the range. At 15 film, KOS-A played a contrary role and increased NO. level for islets both with and without STZ-treatment. Islets insulin secretion changed corresponding to NO. level in the assay. Increased insulin secretion appeared parallel to the decrease of NO., and normal insulin release was not affected by KOS-A less than 1.5 mM. Structure determination of KOS-A shows that it is a tetrasaccharide with Mw of 666 Da and reductive end of alpha-D-mannose. These results indicate that low dosage of KOS-A, with its function on attenuating STZ-induced NO. level, doesn't alter normal NO. and insulin secretion pathways of isolated islets. The NO. attenuation function of KOS-A on the diabetes model is mainly resulted from environmental free radical scavenging by the oligosaccharide. Present results also imply the mechanism of clinical Amorphophallus Konjac hypoglycemic function maybe related with free radical attenuation and lower risks of islets damage from NO. radical. (C) 2002 Elsevier Science Inc. All rights reserved.
KeywordDiabetes Model Streptozotocin Konjac Oligosaccharide Nitric Oxide
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordNITRIC-OXIDE ; VITAMIN-E ; QUANTITATION
WOS Research AreaResearch & Experimental Medicine ; Pharmacology & Pharmacy
WOS SubjectMedicine, Research & Experimental ; Pharmacology & Pharmacy
WOS IDWOS:000179845000008
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5629
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Lu, XJ,Chen, XM,Fu, DX,et al. Effect of Amorphophallus Konjac oligosaccharides on STZ-induced diabetes model of isolated islets[J]. LIFE SCIENCES,2002,72(6):711-719.
APA Lu, XJ,Chen, XM,Fu, DX,Cong, W,&Fan, OY.(2002).Effect of Amorphophallus Konjac oligosaccharides on STZ-induced diabetes model of isolated islets.LIFE SCIENCES,72(6),711-719.
MLA Lu, XJ,et al."Effect of Amorphophallus Konjac oligosaccharides on STZ-induced diabetes model of isolated islets".LIFE SCIENCES 72.6(2002):711-719.
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