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A cone disk atomizer for production of biocompatible magnetic microcapsules for culture of anchorage-dependent mammalian cells
Alternative TitleBiotechnol. Lett.
Lu, XJ; Cong, W; Ouyang, F
2002-05-01
Source PublicationBIOTECHNOLOGY LETTERS
ISSN0141-5492
Volume24Issue:10Pages:825-830
AbstractA cone disk atomizer has been designed for preparation of gelatin/polystyrene magnetic microcapsules for use as microcarriers in anchorage-dependent mammalian cell culture. This apparatus facilitates continuous production of microcapsules and ignores surfactants or organic solvents in traditional emulsion. Compared with the flat-disk atomizer, the feeding flow is subjected to a normal pressure provided by the special cone-shaped disk and liquid slide is dramatically decreased in the cone-disk atomizer. A flow-rate-limitation is observed in both flat-disk atomization and cone-disk atomization. A 36% increase in flow-rate limitation was observed in cone-disk atomization than in flat-disk atomization indicating the potential of this method for large-scale preparations.; A cone disk atomizer has been designed for preparation of gelatin/polystyrene magnetic microcapsules for use as microcarriers in anchorage-dependent mammalian cell culture. This apparatus facilitates continuous production of microcapsules and ignores surfactants or organic solvents in traditional emulsion. Compared with the flat-disk atomizer, the feeding flow is subjected to a normal pressure provided by the special cone-shaped disk and liquid slide is dramatically decreased in the cone-disk atomizer. A flow-rate-limitation is observed in both flat-disk atomization and cone-disk atomization. A 36% increase in flow-rate limitation was observed in cone-disk atomization than in flat-disk atomization indicating the potential of this method for large-scale preparations.
KeywordAtomization Cone-disk Large-scale Magnetic Beads Microcapsules
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000175634500014
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5630
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Lu, XJ,Cong, W,Ouyang, F. A cone disk atomizer for production of biocompatible magnetic microcapsules for culture of anchorage-dependent mammalian cells[J]. BIOTECHNOLOGY LETTERS,2002,24(10):825-830.
APA Lu, XJ,Cong, W,&Ouyang, F.(2002).A cone disk atomizer for production of biocompatible magnetic microcapsules for culture of anchorage-dependent mammalian cells.BIOTECHNOLOGY LETTERS,24(10),825-830.
MLA Lu, XJ,et al."A cone disk atomizer for production of biocompatible magnetic microcapsules for culture of anchorage-dependent mammalian cells".BIOTECHNOLOGY LETTERS 24.10(2002):825-830.
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